Colorectal Neoplasms
Conditions
Brief summary
Fats and oils play important roles in maintaining human nutrition and health through providing energy, essential fatty acids, and acting as modulators of many biological processes (signal transduction, immunity and inflammation). Due to differences in the fatty acid composition and content of antioxidants of individual cooking oils, the degree of oxidative and thermolytic reactions may vary oil by oil. It is lack of human feeding study to investigate the molecular mechanisms on how and which deep-fried oil exerts its adverse effects. The investigators are also lack of biomarkers for monitoring deep-fried oil exposure. Therefore, the purpose of this study is to compare how human body responds differently to several popular uncooked and deep-fried oils with varied fatty acid compositions with respect of oxylipin profile, inflammatory markers, non-targeted metabolomics, and transcriptomics. The investigators will recruit 20 volunteers, provided them once a week the milk shakes prepared from 60g of olive oil, soybean oil, palm oil, camellia oil, tallow (butter), and deep-fried oils of the last 4, respectively; in comparison with a no-fat milk shake control. The experiments lasted for 10 weeks.。Each time; serum, plasma, whole blood and urine samples were collected at baseline, after 2 hours, and after 4 hours. The investigators anticipate to find biomarker(s) for deep-frying, and contribute to the understanding of molecular mechanisms on how deep-fried oils exert adverse effects toward health through integrative omics or so-called system biology approaches.
Interventions
OTHERFat-free milkshake
participants drinks a fat-free milk shakes
OTHEROlive oil
participants drinks the milk shakes prepared from 60g of olive oil
OTHERSoybean oil
participants drinks the milk shakes prepared from 60g of soybean oil
OTHERFried soybean oil
participants drinks the milk shakes prepared from 60g of fried soybean oil
OTHERPalm oil
participants drinks the milk shakes prepared from 60g of palm oil
OTHERFried palm oil
participants drinks the milk shakes prepared from 60g of fried palm oil
OTHERCamellia oil
participants drinks the milk shakes prepared from 60g of camellia oil
OTHERFried camellia oil
participants drinks the milk shakes prepared from 60g of fried camellia oil
OTHERTallow
participants drinks the milk shakes prepared from 60g of tallow
OTHERFried tallow
participants drinks the milk shakes prepared from 60g of fried tallow
Sponsors
Academia Sinica, Taiwan
Study design
Allocation
NON_RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Caregiver)
Eligibility
Sex/Gender
MALE
Age
20 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Non-smoking, non-drinker, usual dietary intake * BMI : 18.5\ 27 kg/m2
Exclusion criteria
1. Over the past two weeks have suffered from acute illness. 2. None of the subjects was taking any supplemental vitamins, antioxidants or medication at that time. 3. Taking steroids or non-steroidal anti-inflammatory drug, such as Aspirin or Panadol in the past one week. 4. Cancer or other severe diseases was diagnosed.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The changs of metabolomics profile between before and after oil consumption | baseline to 2 hours and 4 hours after oil consumption | This is a metabolomics study. We measured retention time (min), mass and abundance of serum metabolites with LC-QTOF at 3 time points. Then, we compared the abundance of metabolites between different oil consumption time ( before and after ) and assessed the health effects of cooking oils. |
Outcome results
None listed