Aging Metabolic Syndrome Gut Microbiome Dysbiosis Hyperglycemia Dyslipidemia Oxidative Stress Inflammation Sarcopenia Gastrointestinal Health Aging Aged Prebiotics Gut Microbiota Wolffia Plant-Based Diet Functional Foods Blood Glucose Insulin Resistance Lipid Metabolism Inflammation Antioxidants Short-Chain Fatty Acids Dietary Fiber
Conditions
Interventions
200 ml daily prebiotic plant-based drink with Wolffia globosa for 7 days. Contains 3 g fiber, protein, amino acids, antioxidants. Given after breakfast. Daily video compliance monitoring.,200 ml daily
Prebiotic Plant-Based Wolffia Drink, Placebo Plant-Based Drink
Sponsors
MU-KMUTT Biomedical Engineering & Biomaterials Research Consortium
Eligibility
Sex/Gender
All
Age
60 Years to 75 Years
Inclusion criteria
Inclusion criteria: Inclusion Criteria For Sensory Evaluation: Males and females aged 60 to 75 years Individuals in stable health without acute illness Capable of understanding instructions and providing informed reliable feedback Willingness to participate Open to consuming plant-based or functional beverages For Clinical Intervention: Males and females aged 60 to 75 years No present or history of diabetes, cardiovascular disease, kidney disease, liver disease, hypertension, gastrointestinal disorder, cancer, food allergy, hypersensitivity, or hematological abnormalities Willing to comply with study protocol Able to provide written informed consent
Exclusion criteria
Exclusion criteria: For Sensory Evaluation: Allergies or intolerances to ingredients in the test product including soy or Wolffia Severe or uncontrolled chronic diseases such as late-stage diabetes, kidney failure, or gastrointestinal disorders Acute illness especially those involving sensory dysfunction including colds, sinus infections, or COVID-19 Diagnosed taste or smell disorders and color vision deficiency Regular smokers, heavy alcohol drinkers, or users of substances that impair taste perception Currently taking medications that alter taste, appetite, or digestion including antibiotics, chemotherapy, or appetite suppressants Cognitive impairment that prevents comprehension or consistent evaluation including advanced dementia For Clinical Intervention: Allergic to ingredients in prebiotic drink with Wolffia Using nutritional supplements over 200% reference nutrient intake or any antioxidant purpose supplement Diarrhea requiring treatment in the 7 days prior to enrollment Current smoking or history of stopping smoking less than 6 months Drinking alcohol in 1 month before or during the study Using antibiotics, steroids, or nonsteroidal anti-inflammatory drugs in 2 months before or during the study Consuming prebiotic or probiotic supplements including yogurt, kimchi, natto, or prebiotic drinks in 2 weeks before or during the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change in Fasting Blood Glucose Baseline (Day 0) and Day 8 Fasting blood glucose measured in mg/dL using standard glucose oxidase assay method. Change calculated as Day 8 value minus baseline Day 0 value. | — |
Secondary
| Measure | Time frame |
|---|---|
| Change in Gut Microbiota Composition Baseline (Day 0) and Day 8 Fecal bacterial populations using fluorescent in situ hybridization (FISH) ,Change in Short-Chain Fatty Acids Baseline (Day 0) and Day 8 Fecal SCFA concentrations (acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, lactate),Change in Serum Lipid Profile Baseline (Day 0) and Day 8 Total cholesterol, triglycerides, HDL, and LDL measured in mg/dL using standard enzymatic methods.,Change in Inflammatory Biomarkers Baseline (Day 0) and Day 8 High-sensitivity C-reactive protein (hs-CRP), TNF-alpha, IL-6, IL-10, and IL-4 measured in serum using ELISA ,Change in Body Composition Baseline (Day 0) and Day 8 Body weight (kg), BMI (kg/m2), waist circumference (cm), hip circumference (cm), fat mass (%), and muscle mass (kg) measured using bioelectrical impedance analysis. | — |
Countries
Thailand
Contacts
Public ContactPattaneeya Prangthip
Faculty of Tropical Medicine, Mahidol University
Outcome results
None listed