Metabolism, Exercise, Dietary Supplements
Conditions
Keywords
amino acids, deuterium oxide, humans, protein synthesis, plant proteins, resistance training, skeletal muscle
Brief summary
Plant-based proteins contain several amino acids that are essential to signal growth, reconditioning, maintenance and preservation of skeletal muscle mass. This study aims to inform on the nutrient activation of muscle protein synthesis (MPS) by feeding a plant protein isolate equivalent to 0.33 g of protein per kilogram of body mass.
Detailed description
Participants: 32 young, healthy men and women aged 18 - 35 years. Intervention: Plant protein isolate (PPI) and nonessential amino acid control (NEAA) Study Design: Block randomised control trial. Two group parallel design. Primary outcome: Myofibrillar fractional synthetic rate (myoFSR). Preliminary screening/familiarization consists of: medical history, dietary assessment and examination by a medical doctor and a qualified dietician, blood sample to be evaluated for health-related contraindications, body composition measurement by dual energy x-ray absorptiometry (DXA), isokinetic knee extensor strength tests. Pre-trial: A metered dose of deuterium oxide (5ml/kg) will be ingested and blood and/or saliva (\ 5ml) samples will be collected pre/post ingestion. Experimental trial: Blood, saliva and bilateral m.vastus lateralis microbiopsies (\ 100 mg) collected. A unilateral resistance-training session consisting of knee extensor contractions will be completed. Following exercise cessation participants will ingest 0.33 g/kg body mass of either: a nonessential amino acid formulation or a plant-protein isolate. A saliva sample and a 2nd set of bilateral m.vastus lateralis microbiopsies are collected 3 h postingestion.
Interventions
DIETARY_SUPPLEMENTNEAA
Food grade mixture of non-essential amino acids in powdered form to be dissolved in water. Supplied by Marigot Ltd, Cork, Ireland
DIETARY_SUPPLEMENTPPI
Food grade plant (legume - Fava) protein isolate to be dissolved in water. Supplied by Marigot Ltd, Cork, Ireland
Sponsors
Marigot Ltd.
Enterprise Ireland
University of Limerick
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Masking description
Single blind (participant)
Intervention model description
Block randomized control trial, parallel group design
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
\- Men and women aged 18 to 35 years
Exclusion criteria
* Known intolerance to any item contained in the protein products. * Blood borne disease risk, current illness or medication that would adversely affect participation * Known adverse reaction to venepuncture and/or biopsy * Inability to undertake resistance exercise * Inability to adhere to protocol guidelines
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Myofibrillar fractional synthetic rate change | 4 hours | The fractional rate of myofibrillar protein synthesis measured by deuterium incorporation into skeletal muscle sampled by microbiopsy |
Countries
Ireland
Outcome results
None listed