Healthy Young, Older People With Cardiovascular Disease Risk
Conditions
Keywords
endothelial function, L-citrulline, nitrate, nitric oxide
Brief summary
Cardiovascular disease is the main cause of death worldwide. Endothelial dysfunction is the first step to development of cardiovascular diseases. Endothelial dysfunction occurs due to reduction in nitric oxide, a molecule that plays an important role on vascular health. Due to important role of nitric oxide in endothelial function, several studies have evaluated the effect of its precursors, such as L-citrulline and nitrate on endothelial function parameters. The present study will evaluate the effect of L-citrulline and nitrate derived by watermelon and beetroot products, respectively, on endothelial function of young and older participants. Furthermore, we will evaluate the plasmatic amino acids, nitrate, glucose and insulin in response to ingestion of these products.
Interventions
DIETARY_SUPPLEMENTWatermelon
A single dose of watermelon powder containing 3g of L-citrulline
DIETARY_SUPPLEMENTBeetroot
A single dose of beetroot powder containing 8 mmol of nitrate
DIETARY_SUPPLEMENTWatermelon + beetroot
A single dose of watermelon and beetroot powder containing 3g of L-citrulline and 8 mmol of nitrate
DIETARY_SUPPLEMENTPlacebo
A single dose of maltodextrin
Sponsors
Universidade Federal do Rio de Janeiro
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 85 Years
Healthy volunteers
Yes
Inclusion criteria
for healthy young participants: * Age between 18-35 years; * Triglycerides ≤ 150 mg/dL * HDL-cholesterol ≥ 50 mg/dL for women and ≥ 40 mg/dL for men; * Waist circumference ≤ 102 cm for men and ≤ 88 cm for women). Inclusion Criteria for older people with cardiovascular risk: * Age ≥ 60 years; * Elevated triglycerides ≥150 mg/dL; * Reduced HDL-cholesterol ≤ 50 mg/dL for women and ≤ 40 mg/dL for men; * Elevated waist circumference ≥102 cm for men and ≥ 88 cm for women).
Exclusion criteria
* Elevated fasting glucose (≥100 mg/dL); * Smoking; * Beetroot or watermelon allergy; * Unwillingness to avoid beetroot or watermelon products during the entire study; * Other chronic diseases (diabetes, liver disease, etc.) or acutely ill
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Flow-mediated dilation (FMD) | This measure will be evaluate before the nutritional intervention |
| Muscle oxygen saturation | This measure will be evaluate before the nutritional intervention |
Secondary
| Measure | Time frame |
|---|---|
| Plasmatic amino acids | This measure will be evaluate before the nutritional intervention |
| Plasmatic glucose | This measure will be evaluate before the nutritional intervention |
| Plasmatic insulin | This measure will be evaluate before the nutritional intervention |
Countries
Brazil
Outcome results
None listed