Inflammation, Oxidative Stress, Vitamin K, Exercise, Strength, Recovery
Conditions
Brief summary
The aim of the current study, therefore, is to investigate the effects of vitamin K2 supplementation on the physiological responses to an acute bout of resistance exercise.
Interventions
DIETARY_SUPPLEMENTVitamin K2
Vitamin K2
DIETARY_SUPPLEMENTMicro-crystalline cellulose
Micro-crystalline cellulose
Sponsors
Kappa Bioscience
University of Glasgow
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Participant is able and willing to sign the Informed Consent From * No plans to change lifestyle (activity and nutrition) during the study period * Older group (n=40): 65 years of age or older. * Younger group (n=40): Aged 18-40 years
Exclusion criteria
* Not currently, or in the last year, participating in more than 1h per week of vigorous aerobic physical activity or any resistance exercise * BMI \> 30 kg/m2 * diabetes * severe cardiovascular disease * seizure disorders * liver disease * uncontrolled hypertension (\>150/90mmHg at baseline measurement) * cancer or cancer that has been in remission \<5 years * ambulatory impairments which would limit ability to perform assessments of muscle function * dementia * currently taking Vitamin K2 supplements * currently taking Vitamin K antagonists/anticoagulants (e.g. warfarin) * current smoking * history of drug abuse * taking medication known to affect muscle (e.g. steroids).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in post exercise recovery of muscle strength | Change from baseline to 12 weeks | Muscle strength (MVC) of the knee extensor muscles will be measured before and after (3h, 24h, 48h, 72h) an acute bout of resistance exercise |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in post exercise recovery of sEMG activity | Change from baseline to 12 weeks | Quadriceps muscle sEMG will be measured before and after (3h, 24h, 48h, 72h) an acute bout of resistance exercise |
| Change in post exercise peroxiredoxin 3 redox state | Change from baseline to 12 weeks | Circulating peroxiredoxin 3 redox state will be measured before and after (3h, 24h, 48h, 72h) an acute bout of resistance exercise |
| Change in vitamin K levels | Change from baseline to 12 weeks | Vitamin K will be measured in baseline and 12 week samples |
| Change in Carboxylated Osteocalcin levels | Change from baseline to 12 weeks | Carboxylated Osteocalcin will be measured in baseline and 12 week samples |
| Change in uncarboxylated Osteocalcin levels | Change from baseline to 12 weeks | uncarboxylated Osteocalcin will be measured in baseline and 12 week samples |
| Change in carboxylated matrix gla-protein levels | Change from baseline to 12 weeks | carboxylated matrix gla-protein will be measured in baseline and 12 week samples |
| Change in dephosphorylated-uncarboxylated matrix gla-protein levels | Change from baseline to 12 weeks | dephosphorylated-uncarboxylated matrix gla-protein will be measured in baseline and 12 week samples |
| Change in post exercise F2 isoprostanes | Change from baseline to 12 weeks | Circulating F2 isoprostanes will be measured before and after (3h, 24h, 48h, 72h) an acute bout of resistance exercise |
| Change in pain free range of motion during knee extension exercise | Change from baseline to 12 weeks | The range of motion which is pain free will be measured during knee extension using a goniometer before and after (3h, 24h, 48h, 72h) an acute bout of resistance exercise |
| Change in post exercise recovery of functional abilities | Change from baseline to 12 weeks | Time to complete 5 chair rises will be measured before and after (3h, 24h, 48h, 72h) an acute bout of resistance exercise |
| Change in post exercise interleukin-6 | Change from baseline to 12 weeks | Circulating interleukin-6 will be measured before and after (3h, 24h, 48h, 72h) an acute bout of resistance exercise |
Other
| Measure | Time frame | Description |
|---|---|---|
| Change in blood lipids | Change from baseline to 12 weeks | Change in blood lipids |
| Change in plasma insulin | Change from baseline to 12 weeks | Change in plasma insulin |
| Change in fat mass | Change from baseline to 12 weeks | Change in fat mass |
| Change in muscle thickness | Change from baseline to 12 weeks | Change in muscle thickness |
| Change in exercise substrate utilisation | Change from baseline to 12 weeks | Energy expenditure, carbodhydrate and fat oxidation during treadmill walking |
| Change in lean mass | Change from baseline to 12 weeks | Change in lean mass |
| Change in blood glucose | Change from baseline to 12 weeks | Change in blood glucose |
Countries
United Kingdom
Outcome results
None listed