Healthy
Conditions
Brief summary
The goal of this project is to use a cross over design in young healthy male and female adults: 1. To determine the amino acid blood response to the acute ingestion of cheddar cheese in comparison to that of milk. 2. To determine the muscle mTORC1 response to the acute ingestion of cheddar cheese in comparison to that of milk.
Detailed description
Each subject will take part in two metabolic studies. Each metabolic study was designed to test the acute blood and muscle response to an ingested amount of either cheddar cheese or milk. Approximately, one month after the first experiment (Metabolic Study #1), the participant will complete the second experiment (Metabolic Study #2) which was exact in design and at the same time of day as the first study but the participant ingested the alternate food product. The morning of the metabolic studies, the participant will arrive at the clinical research center after a 10h fast. A catheter will be placed in the participants' arm for blood sampling. Next, the participant will undergo a fasted thigh skeletal muscle biopsy. Following the muscle biopsy, the participant will consume either cheddar cheese (65g) or milk (370 mL) each amounting to 20g of protein. Subsequent muscle biopsies will occur at 60 and 180min after product ingestion. Blood sampling will occur in the fasted state and periodically after ingestion of the products (up to 300min).
Interventions
DIETARY_SUPPLEMENTCheddar Cheese
20g of protein from cheddar cheese
DIETARY_SUPPLEMENTMilk
20g of protein from milk
Sponsors
University of Utah
Study design
Allocation
NON_RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Participants take part in an experiment in which they consume cheese then at another time consume milk
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Age between 18-35y
Exclusion criteria
1. History of cardiovascular disease 2. History of endocrine or metabolic disease (e.g., hypo/hyperthyroidism, diabetes) 3. History of kidney disease or failure 4. History of liver disease 5. History of respiratory disease (acute upper respiratory infection, chronic lung disease) 6. History of stroke with motor disability 7. Use of anticoagulant therapy (e.g., Coumadin, heparin) including aspirin and fish oils within 7d of the first biopsy experiment 8. Elevated systolic blood pressure \>150 or a diastolic blood pressure \> 100 9. Smoking 10. Recent anabolic or corticosteroids use (within 12 weeks) 11. Pregnancy as determined by a pregnancy test 12. self report of lactose intolerance 13. Any other medical condition or event considered exclusionary by the PI and faculty physician
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Response to cheese or milk ingestion | Area under the curve 0-300min after ingestion | blood amino acids area under the curve in response to acute stimulation of cheese or milk consumption |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Response to cheese or milk ingestion | Change between 60min after ingestion and fasted response | muscle mTORC1 change in response to acute stimulation of cheese or milk |
Countries
United States
Outcome results
None listed