Healthy Adults
Conditions
Keywords
Interesterified, Postprandial, Lipid
Brief summary
The purpose of this study is to investigate whether there are differences in postprandial cardiometabolic metabolic indices following interesterified fats used commercially versus application appropriate non- interesterifed alternative fat.
Detailed description
Aim: The current study aims to investigate the acute effects of commercially available interesterified fat versus versus application appropriate non- interesterifed alternative fat on 8 h postprandial cardiometabolic outcomes. Hypothesis: Interesterified fat will induce a different cardiomtabolic response compared to a non interesterifed, application appropriate fat. Subjects: Participants will include 40 healthy male and female volunteers aged between the ages of 35 and 75 years (since during this age metabolic changes start to take place). Power calculation: Based on previous studies carried out by our group at King's College London, a sample size of 20 participants (males and females separately) will allow a 2.16% (percentage unit) difference in FMD to be detected (90% power, 0.05 alpha, SD 2.9. 50 participants will be recruited allowing for a 20% dropout rate. Expected value:The study will provide novel information on the acute effects of commercially relevant processed fats on postprandial lipaemia.
Interventions
DIETARY_SUPPLEMENTInteresterified fat
50 g fat provided as commercially available interesterified fat.
DIETARY_SUPPLEMENTNon- interesterified fat
50 g fat provided as commercially available non-interesterified fat.
DIETARY_SUPPLEMENTRapeseed oil
50 g fat provided as commercially available rapeseed oil.
DIETARY_SUPPLEMENTButter
50g fat provided as commercially available spreadable butter
Sponsors
King's College London
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
35 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Males and females aged 35-75 years * Healthy (free of diagnosed diseases listed in
Exclusion criteria
) * Able to understand the information sheet and willing to comply with study protocol * Able to give informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Endothelial function | Up to 8 hours | Flow mediated dilatation |
| Postprandial lipaemic response | Up to 8 hours | Area under plasma TAG concentration/ time curve |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Glycaemic response | Up to 8 hours | Plasma glucose concentration |
| Insulinaemic response | Up to 8 hours | Plasma insulin concnetration |
| Postprandial lipaemic response | Up to 8 hours | Postprandial plamsa total fatty acid composition and non esterifed fatty acid |
| Oxidative stress | Up to 8 hours | Plasma 8-isoprostane F2a |
| Inflammatory response | Up to 8 hours | Plasma IL-6 |
| Nitric oxide bioavailability | Up to 8 hours | Plasma nitric oxide species and NADPH oxidase activity |
| Metabolomic for assessment of atherogenic potential (lipoprotein) analysis | Up to 8 hours | Lipid concentrations and compositions of 14 lipoprotein subclasses, measured by NMR |
Countries
United Kingdom
Outcome results
None listed