Healthy Adults, Postmenopausal Women
Conditions
Keywords
Interesterified, Postprandial, Lipid
Brief summary
The purpose of this study is to investigate whether there are differences in postprandial metabolic indices following interesterified fats used commercially versus the corresponding un-interesterified blend.
Detailed description
Aim: The current study aims to investigate the acute effects of commercially relevant interesterified 'hardstock' versus the corresponding un-interesterified blend on 8 h postprandial fat metabolism. Hypothesis: Interesterification of a palm kernel and palm stearin fat blend, to produce a fat with a higher proportion of palmitic acid in the middle position of the triglyceride (TAG; but the same fatty acid composition), will alter postprandial lipid metabolism. Subjects: Participants will include 24 healthy male and female (postmenopausal) volunteers aged between the ages of 45 and 75 years (since during this age metabolic changes start to take place). Power calculation: Based on previous studies carried out by our group at King's College London, a sample size of 10 males and 10 females is required to detect a (clinically relevant) difference (for males and females separately) between means of 0.3 mmol/L maximal difference in 8 h plasma TAG concentration (standard deviation 0.33 mmol/L, significance level (alpha) of 0.05 and 80% power). 12 males and 12 females will be recruited to allow for a 20% drop out rate. Expected value:The study will provide novel information on the acute effects of commercially relevant processed fats on postprandial lipaemia.
Interventions
DIETARY_SUPPLEMENTInteresterified blend of palm kernal and plam stearin
50 g fat provided as interesterified palm kernal and palm sterin blend in a single meal (sub-group, n=12, also have 75 mg 13C labelled tripalmatin and 1.2 g / kg body water deuterated water)
DIETARY_SUPPLEMENTUn-interesterified blend of palm kernal and plam stearin
50 g fat provided as un-interesterified palm kernal and palm sterin blend in a single meal (sub-group, n=12, also have 75 mg 13C labelled tripalmatin and 1.2 g / kg body water deuterated water)
DIETARY_SUPPLEMENTRapeseed oil
50 g fat provided as rapeseed oil in a single meal (sub-group, n=12, also have 75 mg 13C labelled tripalmatin and 1.2 g / kg body water deuterated water)
Sponsors
King's College London
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
45 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Males and females aged 45-75 years * Healthy (free of diagnosed diseases listed in
Exclusion criteria
) * Able to understand the information sheet and willing to comply with study protocol * Able to give informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Area under plasma TAG concentration/ time curve | Up to 8 hours | Postprandial area under plasma TAG concentration/ time curve |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Postprandial lipaemic response | Up to 8 hours | Postprandial plamsa total fatty acid composition and non esterifed fatty acid |
| Positional composition retention (chylomicron) | Up to 6 hours | Postprandial chylomicron TAG concentration and composition, TAG sn-2 fatty acid composition, total protein, apoB100 and apoB48 |
Other
| Measure | Time frame | Description |
|---|---|---|
| Isotope lablelled parameters | Up to 8 hours | In sub-group, n=12, postprandial 13C TAG concentration and breath CO2 13C |
| Lipoprotein particle size and number | Up to 8 hours | Lipoprotein size (LDL, VLDL and HDL) and composition (total and small LDL, large VLDL and total and large HDL) measured by NMR |
| 2 and 3 MCPD and glycidyl esters | Up to 8 hours | Postprandial plasma 2 and 3-monochloropropane-1,2-diol or 3-chloropropane-1,2-diol glycidyl esters |
Countries
United Kingdom
Outcome results
None listed