Insulin Resistance
Conditions
Brief summary
The aim of the study will investigate whether impairment in the action of insulin to promote the use of glucose in skeletal muscle (insulin resistance) as a result of oral ingestion of a liquid meal rich in saturated fat is linked to reduced ability of muscles to synthesise new protein in response to dietary protein intake, which ultimately may compromise maintenance of muscle size and quality of life and whether partially replacing saturated fat in the liquid meal with omega 3 polyunsaturated FA (n3PUFA) will ameliorate these negative effects.
Interventions
DIETARY_SUPPLEMENTSaturated fat
Palm stearin
DIETARY_SUPPLEMENTPlacebo
Water
DIETARY_SUPPLEMENTFish Oil
Fish Oil, Omega 3 PUFA
Sponsors
University of Nottingham
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
40 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Male, healthy, overweight (BMI\>27 kg/m2 and fat mass \>30%) non-diabetic individuals.
Exclusion criteria
* Diabetes, smoking, cardiovascular disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in muscle protein fractional synthetic rate | 3 hours post protein drink | Measurement of FSR at baseline and post intervention |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| whole body postprandial insulin sensitivity | Baseline and every 15mins for 7 hours | Glucose and insulin area under the curves (AUC) in response to the milk protein and glucose ingestion will be measured |
| Whole body and skeletal muscle fat oxidation | Baseline and every 15mins for 7 hours | D31palmitate plasma enrichment and incorporation into skeletal muscle lipids |
Outcome results
None listed