Healthy
Conditions
Keywords
protein quality
Brief summary
Our objective is to determine the metabolic availability of Tryptophan in white maize using the indicator amino acid oxidation (IAAO) technique in adult men.
Detailed description
Our objective is to determine the metabolic availability of Tryptophan in white maize. Seven young, healthy, male adults will receive graded levels (13.2, 26.4, 39.7, 52.9) of tryptophan requirement of 3.78 mg/kg/d as a crystalline amino acid (AA) mixture and a porridge of white cornmeal protein respectively.
Interventions
OTHERTryptophan
Tryptophan will be supplied at 13.2, 26.4, 39.7, or 52.9% of requirement; sourced from crystalline amino acid and white cornmeal maize.
Sponsors
The Hospital for Sick Children
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
HEALTH_SERVICES_RESEARCH
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
20 Years to 49 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy Adult males (20-49years old). * Stable body weight * Not on any medications that could affect protein and amino acid metabolism e.g. steroids.
Exclusion criteria
* Recent weight loss within the last 3 months or on weight reducing diet. * Unwillingness to participate or unable to tolerate the diet.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic Availability of Tryptophan in Corn | 9 hours | The quality of corn protein will be assessed by measuring the amount of its tryptophan available for protein synthesis (metabolic availability) using the slope ration method. The oxidation of the indicator amino acid phenylalanine will be measured in response to feeding graded intakes of tryptophan in a reference protein patterned after egg protein, and provided as a crystalline amino acid mixture. The pattern of oxidation of phylalanine in response to tryptophan provided in corn will be compared to the pattern of oxidation obtained from feeding the reference protein. On each study day, subjects will be fed 8 hourly meals and breath samples will be taken to measure the oxidation of phenylalanine. Samples will be collected at baseline after the fourth meal, and half hourly, beginning 2.5 hrs after the 5th meal. Breath samples will be analyzed for 13CO2 enrichment. |
Countries
Canada
Outcome results
None listed