Short ejaculatory abstinence less than 48 hours with standard abstinence between 2 to 7 days on SDF levels and fertilization outcomes in in vitro fertilization (IVF) cycles Short ejaculatory abstinence, Sperm DNA fragmentation, Fertilization rate, In vitro fertilization (IVF) cycles, Blastocyst rate
Conditions
Interventions
Participants will be instructed to maintain ejaculatory abstinence for less than 48 hours prior to semen collection (shortened ejaculatory abstinence),Participants will be instructed to maintain ejacu
Experimental Behavioral,Active Comparator Behavioral
Shortened ejaculatory abstinence,Conventional ejaculatory abstinence
Sponsors
Faculty of Medicine, Khon Kaen University
Eligibility
Sex/Gender
All
Age
20 Years to 45 Years
Inclusion criteria
Inclusion criteria: Male partner 1.Male partner aged 20 to 45 years with infertility, attending for in vitro fertilization treatment at the Infertility Clinic, Srinagarind Hospital. 2.No history of testicular or penile surgery, or previous vasectomy. 3.No history of cancer, radiotherapy, or chemotherapy. Female partner 1.Female partner aged 20 to 40 years. 2.AntiMullerian hormone level equal or more than 1.2 ng per mL or antral follicle count equal or more than 5 follicles.
Exclusion criteria
Exclusion criteria: Male partner 1.Inability to produce semen by masturbation or requiring surgical sperm retrieval. 2.Severe oligozoospermia, defined as sperm concentration more than 5 million sperms/mL, or azoospermia. 3.Current cigarette smoking more than 20 cigarettes/day, or smoking cessation within the past 3 months. 4.Heavy alcohol intake, defined as more than 80 g/day, equivalent to equal or more than 8 standard drinks/day, for example 6 to 8 cans of beer/day or 5 to 6 shots of liquor/day. 5.Presence of varicocele or other forms of genital heat stress, including high-grade fever or external heat exposure such as sauna, hot bath, working in a high temperature environment, prolonged sitting or driving for equal or more than 2 hours per session, or regular use of very tight underwear or tight trousers. 6.Inflammation or sexually transmitted infection of the male reproductive tract. 7.Metabolic abnormalities, such as impaired glucose tolerance. 8.Obesity, defined as body mass index (BMI) more than 30 kilograms per square meter. Female partner 1.History of endometriosis.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Sperm DNA Fragmentation (SDF) Level/index After semen collection (before and after preparation) Percentage of sperm DNA fragmentation measured by validated SDF assay comparing abstinence less than 48 hours with 2 to 7 days,Number of metaphase II oocytes After intracytoplasmic sperm injection Count metaphase II oocytes after intracytoplasmic sperm injection, comparing shortened versus conventional ejaculatory abstinence ,Fertilization rate After intracytoplasmic sperm injection Fertilization rate defined as proportion of 2PN oocytes per total inseminated or injected oocytes comparing shortened versus conventional abstinence,Number of cleavage stage embryos After intracytoplasmic sperm injection day 3 Count embryos in cleavage stage in day 3 after intracytoplasmic sperm injection, comparing shortened versus conventional ejaculatory abstinence,Number of blastocyst embryos After intracytoplasmic sperm injection day 5 Count blastocyst embryos after intracytoplasmic sperm injection day 5, comparing shortened versus conventional ejaculatory abstinence ,Blastocyst Formation and Embryo Quality After intracytoplasmic sperm injection day 5 Number of blastocysts formed and embryo quality assessed using standard morphological grading criteria, comparing shortened versus conventional ejaculatory abstinence | — |
Secondary
| Measure | Time frame |
|---|---|
| Basic Semen Parameters After semen collection (before and after preparation) Semen volume concentration count motility and morphology assessed by WHO semen manual 6th edition comparing shortened versus conventional abstinence | — |
Countries
Thailand
Contacts
Public ContactAchara Pollahan
Faculty of Medicine, Khon Kaen University
Outcome results
None listed