The Effect of Local Food-Based Intervention between Nile Tilapia (Oreochromis Niloticus) and Banana Blossom (Musa Balbisiana Colla.) on Lactoferrin Levels for Breastfeeding Mothers

Effect of a Local Food-Based Intervention Using Nile Tilapia (Oreochromis niloticus) and Banana Blossom (Musa balbisiana Colla.) on Lactoferrin Levels in Breastfeeding Mothers: A Quasi-Experimental Study

Status

Active, not recruiting

Phases

Phase 2

Study type

Interventional

Source

TCTR

Registry ID

TCTR20260424011

Enrollment

Registered

2026-04-24

Start date

2025-04-29

Completion date

Unknown

Last updated

2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Low lactoferrin levels in breast milk Breastfeeding mothers Infant immune function Breast milk quality Lactoferrin Breast Feeding Human Milk Dietary Intervention Maternal Nutrition Infant Immunity Protein Intake Iron Micronutrients

Interventions

Participants received a daily dietary intervention consisting of 200 g of processed Nile tilapia (Oreochromis niloticus) and 200 g of processed banana blossom (Musa balbisiana) for 14 consecutive days

Experimental Dietary Supplement,No Intervention No treatment

Local food-based intervention (tilapia and banana blossom),Breastfeeding promotion only

Eligibility

Sex/Gender

Female

Age

20 Years to 35 Years

Inclusion criteria

Inclusion criteria: 1. Breastfeeding mothers at 14 days postpartum 2. Practicing exclusive breastfeeding 3. Willing to participate and provide informed consent 4. Having a normal body mass index (BMI) 5. Free from infections and postpartum complications 6. Residing in the study area

Exclusion criteria

Exclusion criteria: 1. Participants who withdrew from the study 2. Participants who did not complete the intervention protocol 3. Mothers who developed infections or postpartum complications during the study period

Design outcomes

Primary

Measure	Time frame
Serum lactoferrin levels Baseline (day 13 postpartum) and post-intervention (day 28 postpartum) Measured using enzyme-linked immunosorbent assay (ELISA) from serum samples, expressed in ng/mL	—

Secondary

Measure	Time frame
Energy intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Protein intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Fat intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Vitamin A intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Vitamin B12 intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Iron intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Calcium intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Zinc intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA)	—

Measure

Time frame

Energy intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Protein intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Fat intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Vitamin A intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Vitamin B12 intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Iron intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Calcium intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA),Zinc intake adequacy During intervention period Assessed using two 24-hour dietary recalls and expressed as a percentage of the recommended dietary allowance (%RDA)

—

Countries

Indonesia

Contacts

Public ContactMohammad Rahfiludin

Doctoral Program in Public Health, Faculty of Public Health, Universitas Diponegoro, Semarang, Indonesia

rahfiludinzen@gmail.com08122889745

Outcome results

None listed

Source: TCTR (via WHO ICTRP) · Data processed: Aug 10, 2026