Female infertility Progestin primed ovarian stimulation Embryo morphokinetics Time lapse imaging
Conditions
Interventions
Participants will undergo controlled ovarian stimulation with recombinant FSH. GnRH antagonist will be initiated using a flexible approach when at least one follicle reaches 14 mm and continued until
Active Comparator Drug,Experimental Drug
GnRH antagonist protocol,Progestin primed ovarian stimulation protocol
Sponsors
Faculty of Medicine, Chulalongkorn University
Eligibility
Sex/Gender
Female
Age
20 Years to 43 Years
Inclusion criteria
Inclusion criteria: 1.Female patients with infertility aged 20 to 43 years 2.Undergoing IVF ICSI treatment with freeze all protocol and planned frozen blastocyst transfer 3.Planned preimplantation genetic testing for aneuploidy (PGT A) 4.Planned ovarian stimulation with either GnRH antagonist protocol or progestin primed ovarian stimulation protocol 5.Antral follicle count more than 5 on cycle day 2 to 3 6.Able and willing to provide written informed consent
Exclusion criteria
Exclusion criteria: 1.Basal FSH more than 10 IU per L or AMH less than 0.5 ng per mL 2.Diagnosed endometriosis rASRM grade III or higher 3.Diagnosed polycystic ovary syndrome 4.Functional ovarian cyst with estradiol level more than 100 pg per mL 5.Poor ovarian response with 3 or fewer follicles suitable for oocyte retrieval on trigger day 6.Use of hormonal treatment within the past 3 months 7.Medical contraindication to ovarian stimulation treatment
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Time to blastocyst During embryo culture up to day 7 Time to blastocyst formation measured by time lapse imaging EmbryoScope Plus in hours from insemination or ICSI to full blastocyst stage | — |
Secondary
| Measure | Time frame |
|---|---|
| Morphokinetic parameters During embryo culture up to day 7 Embryo morphokinetic time points measured by time lapse imaging EmbryoScope Plus including pronuclear appearance and cleavage timings and blastulation,Number of oocytes retrieved Day of oocyte retrieval Total number of oocytes retrieved at oocyte pick up per participant,Number of mature oocytes Day of oocyte retrieval Number of metaphase II oocytes assessed after denudation,Blastocyst formation rate During embryo culture up to day 7 Number of blastocysts divided by number of normally fertilized embryos,Embryo quality Day 5 to day 7 embryo assessment Blastocyst morphology grading assessed by embryologists according to laboratory standard criteria,Euploid embryo rate After PGT result available Proportion of euploid embryos among biopsied blastocysts by PGT for aneuploidy,iDAScore During embryo culture up to day 7 Artificial intelligence based embryo score generated by time lapse system,Clinical pregnancy rate At 6 to 7 weeks of gestation Presence of intrauterine gestational sac with fetal heartbeat on ultrasound after frozen euploid blastocyst transfer,Embryo morphological data During embryo culture day 5 to day 7 Embryo morphology assessment and blastocyst grading recorded from time lapse images according to laboratory standard criteria including inner cell mass grade and trophectoderm grade | — |
Countries
Thailand
Contacts
Public ContactTeerapat Aiemsamran
Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University
Outcome results
None listed