DNA repair gene expression in cumulus cells of mature oocytes: A comparative study between young and advanced-age women

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

TCTR

Registry ID

TCTR20260118002

Enrollment

Registered

2026-01-18

Start date

2026-03-01

Completion date

Unknown

Last updated

2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Infertility DNA repair Gene expression Advanced maternal age Transcriptomics Cumulus cells

Interventions

Age less than 35 years old,Age 35 years old and more

Basic Science,Basic Science

Young age,Advanced age

Eligibility

Sex/Gender

Female

Age

25 Years to No maximum

Inclusion criteria

Inclusion criteria: 1. Women diagnosed with infertility who are undergoing treatment with in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). 2. Women receiving ovarian stimulation using a GnRH antagonist protocol, including stimulation with recombinant follicle-stimulating hormone (recombinant FSH), prevention of premature ovulation with a GnRH antagonist, and final oocyte maturation triggered by a dual-trigger approach using a GnRH agonist and recombinant human chorionic gonadotropin (hCG).

Exclusion criteria

Exclusion criteria: 1. Women diagnosed with conditions that may affect gene expression, such as endometriosis, polycystic ovary syndrome (PCOS), or endocrine disorders (e.g., diabetes mellitus). 2. Women with a body mass index (BMI) more than 30 kg/m2. 3. Women with a history of ovarian surgery.

Design outcomes

Primary

Measure	Time frame
DNA repair gene expression in Cumulus cell of MII oocytes between younger and older patients 12 months DNA repair gene expression was assessed by RNA sequencing to identify differentially expressed genes between two groups, and results were validated using RT-qPCR.	—

Secondary

Measure	Time frame
Age-related alterations in DNA repair pathways for evaluating ovarian aging. 12 months To investigate which DNA repair mechanisms are primarily associated with age-related changes in order to explain oocyte aging.,Oocyte quality, such as fertilization and blastocyst development. 12 months To investigate the association between DNA repair gene expression and oocyte quality, such as fertilization and blastocyst development (in percentage )	—

Measure

Time frame

Age-related alterations in DNA repair pathways for evaluating ovarian aging. 12 months To investigate which DNA repair mechanisms are primarily associated with age-related changes in order to explain oocyte aging.,Oocyte quality, such as fertilization and blastocyst development. 12 months To investigate the association between DNA repair gene expression and oocyte quality, such as fertilization and blastocyst development (in percentage )

—

Countries

Thailand

Contacts

Public ContactTanaporn Kuwawattananont

Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University

porportanaporn@gmail.com022564829

Outcome results

None listed

Source: TCTR (via WHO ICTRP) · Data processed: Aug 10, 2026