Effect of Sea Cucumber Based Biscuit on Iron Status and Bone Growth in Adolescent Girls with Iron Deficiency Anemia

Effect of a Sea Cucumber Based Food Intervention on Erythrocyte Hematological Profile, Soluble Transferrin Receptor, Osteocalcin, and Linear Growth among Adolescent Girls with Iron Deficiency Anemia: A Controlled Trial

Status

Active, not recruiting

Phases

Phase 3

Study type

Interventional

Source

TCTR

Registry ID

TCTR20260117001

Enrollment

Registered

2026-01-17

Start date

2025-01-01

Completion date

Unknown

Last updated

2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Iron deficiency anemia Iron deficiency anemia Impaired bone metabolism

Interventions

Participants in the intervention group received 100 g/day of Holothuria scabra based biscuits (two sweet and two savory variants) for 12 weeks, consumed five days per week during school days.,Particip

Experimental Dietary Supplement,Active Comparator Dietary Supplement

Intervention Group,Control Group

Eligibility

Sex/Gender

Female

Age

12 Years to 14 Years

Inclusion criteria

Inclusion criteria: 1. Female adolescents aged 12 14 years 2. Hemoglobin concentration between 8.0 and 11.9 g dL 3. No history of thalassemia 4. No known allergy to seafood 5. Non smokers

Exclusion criteria

Exclusion criteria: 1. Failure to complete the study procedures 2. Withdrawal of consent during the study period 3. Presence of severe illness during the study 4. Irregular menstrual cycle

Design outcomes

Primary

Measure	Time frame
Hemoglobin concentration Mean corpuscular volume (MCV) Mean corpuscular hemoglobin (MCH) Red cell distribution width (RDW) At baseline and after 12 weeks of intervention. Hemoglobin and red blood cell indices were measured using an automated hematology analyzer (Sysmex XN-350). ,Serum osteocalcin and soluble transferrin receptor (sTfR) levels At baseline and after 12 weeks of intervention. Serum osteocalcin and soluble transferrin receptor levels were measured using enzyme-linked immunosorbent assay (ELISA).,Body height At baseline and after 12 weeks of intervention. Body height was measured using a standardized stadiometer.,Height-for-age Z-score (HfA) At baseline and after 12 weeks of intervention. Height-for-age Z-score was calculated using WHO AnthroPlus software.	—

Measure

Time frame

Hemoglobin concentration Mean corpuscular volume (MCV) Mean corpuscular hemoglobin (MCH) Red cell distribution width (RDW) At baseline and after 12 weeks of intervention. Hemoglobin and red blood cell indices were measured using an automated hematology analyzer (Sysmex XN-350). ,Serum osteocalcin and soluble transferrin receptor (sTfR) levels At baseline and after 12 weeks of intervention. Serum osteocalcin and soluble transferrin receptor levels were measured using enzyme-linked immunosorbent assay (ELISA).,Body height At baseline and after 12 weeks of intervention. Body height was measured using a standardized stadiometer.,Height-for-age Z-score (HfA) At baseline and after 12 weeks of intervention. Height-for-age Z-score was calculated using WHO AnthroPlus software.

—

Secondary

Measure	Time frame
Serum 25-hydroxyvitamin D level At baseline and after 12 weeks of intervention. Serum 25-hydroxyvitamin D level was measured using enzyme-linked immunosorbent assay (ELISA).	—

Countries

Indonesia

Contacts

Public ContactAndi Sulistianingsi

Universitas Diponegoro

andirispahris@gmail.com081347395582

Outcome results

None listed

Source: TCTR (via WHO ICTRP) · Data processed: Aug 10, 2026