Gut microbiota in obesity and type 2 diabetes. Obesity, type 2 diabetes mellitus, metabolic biomarkers, gut microbiota, Triphala extract
Conditions
Interventions
The 2,000 mg of herbal extract will be used for treatment in the human gut model during 48 hours of fermentation time under controlled conditions: pH, temperature, and anaerobic.
Experimental Dietary Supplement
Sponsors
National Research Council of Thailand (NCRT)
Fundamental fund Kasetsart University
Eligibility
Sex/Gender
Female
Age
20 Years to 60 Years
Inclusion criteria
Inclusion criteria: 1.Normal weight (BMI 18.5 to 24.9 kg/m2) 2.Obese (BMI more than 30 kg/m2) 3.T2DM - normal weight (Blood sugar level higher than 126 mg/dL, and BMI 18.5 to 24.9 kg/m2) 4.T2DM - Obese (Blood sugar level higher than 126 mg/dL, and BMI more than 30 kg/m2)
Exclusion criteria
Exclusion criteria: 1.History of intestinal surgery 2.Pregnancy/breastfeeding 3.The use of antibiotics and prebiotics within 3 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The comparison of gut microbiota composition and metabolic activity before and after the intervention The gut microbiota will be analyzed by the shotgun metagenomic sequencing technique, and metabolic activity will be analyzed by metaproteomic and metabolomic analysis | — |
Secondary
| Measure | Time frame |
|---|---|
| The relationship between gut microbiota composition and metabolic activity before and after the intervention The integration of multi-omics data will be performed to proceed and visualize the relationships or correlations between gut microbiota and metabolic activity | — |
Countries
Thailand
Contacts
Public ContactPaiboon Tunsagool
Biot KU
Outcome results
None listed