Health effects of cigarette smoke exposure in healthy Thai adults, including active smokers and secondhand smoke-exposed individuals. Vegetables Antioxidants Oxidative Stress Detoxification Proteomics Metabolomics Tobacco Smoke Pollution Smoking Environmental Exposure Biomarkers Adult Thailand
Conditions
Interventions
Participants in this study will consume fresh watercress daily for a specified period. The intervention aims to evaluate the effects of watercress consumption on detoxification, antioxidant capacity a
Watercress Intake (Non-Smoker),Watercress Intake (Non-Smoker exposed to cigarette smoke),Watercress Intake (Smoker)
Sponsors
Research Institute for Health Sciences, Chiang Mai University and Center for Global Health and Social Responsibility, University of Minnesota
Eligibility
Sex/Gender
All
Age
20 Years to 60 Years
Inclusion criteria
Inclusion criteria: 1. Healthy adults living in Chiang Mai 2. Eligible participants will be generally 3. (i) Daily smokers of at least 5 cigarettes per day for at least 1 year, not planning to quit, or (ii) non-smokers (< 100 cigarettes over lifetime, none within 1 year prior to screening), exposed to SHS at home or at work at least once weekly, or (iii) non-smokers with no SHS exposure
Exclusion criteria
Exclusion criteria: 1. Individuals with severe underlying medical conditions diagnosed by a physician, 2. Pregnant or breastfeeding women. 3. Individuals with conditions that impair cooperation, such as psychiatric disorders, drug addiction, or alcoholism. 4. Individuals who refuse to participate or request to withdraw from the study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Detoxification At baseline or before starting watercress consumption and Post-intervention or after 7 consecutive days of watercress consumption NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol) urinary excretion levels ,Antioxidant capacity and oxidative stress marker At baseline or before starting watercress consumption and Post-intervention or after 7 consecutive days of watercress consumption Total Antioxidant Capacity (TAC), Enzymatic activity and malondialdehyde (MDA) levels | — |
Secondary
| Measure | Time frame |
|---|---|
| Oxidative DNA damage and acrolein DNA adducts At baseline or before starting watercress consumption and Post-intervention or after 7 consecutive days of watercress consumption Levels of DNA adducts 8-oxo-dG and M1dG in blood DNA and acrolein adduct levels in oral cells,Proteomic and Metabolomic analysis At baseline or before starting watercress consumption and Post-intervention or after 7 consecutive days of watercress consumption Changes in protein expression and metabolic pathways will be analyzed using proteomic and metabolomic techniques. | — |
Countries
Thailand
Contacts
Public ContactKanokwan Kulprachakarn
Research Institute for Health Sciences, Chiang Mai University
Outcome results
None listed