Buccal single-cell analysis in moderate to severe house dust mite-induced allergic rhinitis receiving sublingual immunotherapy
Conditions
Interventions
Lebel score decrease equal or more than 3 after 4 months of SLIT,Lebel score decrease less than 3 after 4 months of SLIT
Basic Science,Basic Science
Responder ,Non-responder
Sponsors
Chulalongkorn University
The Allergy, Asthma &
Immunology Association of Thailand (AAIAT)
Eligibility
Sex/Gender
All
Age
18 Years to 60 Years
Inclusion criteria
Inclusion criteria: 1.House dust mite-induced allergic rhinitis (According to the ARIA definition) Sensitization to house dust mite (Any one of two tests) - Wheal size of skin prick test to Dp or Df >= 3 mm - serum Der p-specific IgE or Der f-specific IgE >0.35 kUA/L 2.Moderate to severe symptoms (According to the ARIA definition) 3.Patients are willing to undergo SLIT
Exclusion criteria
Exclusion criteria: 1.Asthma 2.FEV1 < 70% predicted 3.Pregnancy 4.Have gotten AIT in the past 5 years 5.Nasal surgery within 3 months 6.on immunosuppressive drugs 7.Cancer 8.Systemic diseases effecting immune system such as diabetes mellitus, chronic liver disease, chronic kidney disease, malnutrition etc. 9.Smoking 10.Regular alcohol drinking (current light to heavier drinker) 11.Dental carries, chronic ulcer or other diseases of oral or buccal mucosa 12.Cosensitization with seasonal allergen, pet allergen 13.Food allergy 12.Bleeding tendency (Including the use of antiplatelet and anticoagulant)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Gene expression in oral mucosal cells between responders and non-responders Pre-treatment peroid Single-cell RNA sequencing | — |
Secondary
| Measure | Time frame |
|---|---|
| Changes in gene expression in oral mucosal cells before and after HDM SLIT Pre-treatment and Post-treatment periods Single-cell RNA sequencing | — |
Countries
Thailand
Contacts
Public ContactHiroshi Chantaphakul
Chulalongkorn university
Outcome results
None listed