Obesity Prebiotics, Intestinal Microbiota
Conditions
Interventions
Group A consumed 10 g of inulin extracted from Thai Jerusalem artichoke by our patent technique (Patent no. 15858) administered once daily before dinner.,Group B received isocaloric maltodextrin.,Grou
Experimental Dietary Supplement,Placebo Comparator Dietary Supplement,Active Comparator Dietary Supplement
Inulin,Maltodextrin,Dietary fiber
Sponsors
The Ratchadapisek Somphot Fund
NSTDA Research Fund
Eligibility
Sex/Gender
All
Age
7 Years to 15 Years
Inclusion criteria
Inclusion criteria: 1. Children, age 7 to 15 years 2. Body mass index (BMI) is equal to or greater than median + 2 Standard deviations (SDs)
Exclusion criteria
Exclusion criteria: 1. Underlying disease of syndromic obesity and monogenic obesity 2. Endocrine causes of obesity (e.g. hypothyroidism, growth hormone deficiency) 3. Use of drugs that influence appetite or body weight (e.g. corticosteroids) 4. Attending other concurrent weight reduction programs
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Gut microbiota From the enrollment to the end of intervention at 6 months 16S rRNA gene sequencing | — |
Secondary
| Measure | Time frame |
|---|---|
| Body mass index (BMI) z-score From the enrollment to monthly visit and the end of intervention at 6 months BMI was calculated as weight in kilograms divided by the square of height in meters (kg/m2). The BMI z-score was calculated based on the WHO growth reference.,Fat mass index (FMI), fat-free mass index (FFMI), Trunk FMI, and visceral fat area From the enrollment to the end of intervention at 6 months FMI and FFMI were calculated. Trunk FMI and visceral fat area were measured using standard clinical techniques. ,Inflammatory cytokines (Interleukin-1 beta, Tumor Necrosis Factor-alpha, and Interleukin-6) From the enrollment to the end of intervention at 6 months The ELISA method (Enzyme-Linked Immunosorbent Assay),Children Eating Behaviors From the enrollment to the end of intervention at 6 months Children Eating Behavior Questionnaires (CEBQs),Serum GLP-1 hormone From enrollment to the end of intervention at 6 months The ELISA method (Enzyme-Linked Immunosorbent Assay),PYY hormone From enrollment to the end of intervention at 6 months The ELISA method (Enzyme-Linked Immunosorbent Assay),Myokine (interleukin-15) From enrollment to the end of intervention at 6 months The ELISA method (Enzyme-Linked Immunosorbent Assay),Creatinine/cystatin C ratio From enrollment to the end of intervention at 6 months Serum creatinine is measured using an enzymatic method on the Alinity C analyzers (mg/dL), and cystatin C assessed using particle-enhanced immunonephelometry using the BN Systems (mg/L).,Amino acids and biogenic amines From the enrollment to the end of intervention at 6 months Liquid Chromatography - Mass Spectrometry (LC-MS/MS),Gut Microbiome Enterotype Classification From the enrollment to the end of intervention at 6 months A robust clustering approach combining Bray-Curtis dissimilarity (Hierarchical Clustering) and NMF-transformed loadings (Spectral Clustering) | — |
Countries
Thailand
Contacts
Public ContactChonnikant Visuthranukul
Faculty of Medicine, Chulalongkorn University
Outcome results
None listed