Infertility couple performing ovarian stimulation and preimplantation genetic testing apoptosis, gene expression, transcriptomics, spent culture media, blastocyst
Conditions
Interventions
Euploid blastocyst confirmed by preimplantation genetic testing for aneuploidy (PGT-A),Aneuploid blastocyst confirmed by preimplantation genetic testing for aneuploidy (PGT-A)
Diagnostic,Diagnostic
Euploid blastocyst,Aneuploid blastocyst
Sponsors
King Chulalongkorn Memorial Hospital
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: 1. Infertile women undergoing in vitro fertilization (IVF/ICSI) with blastocyst undergoing preimplantation genetic testing (PGT-A) 2. GnRH antagonist ovarian stimulation protocol with Dual trigger for final oocyte maturation
Exclusion criteria
Exclusion criteria: 1. Abnormal intrauterine cavity which may affect pregnancy rate e.gland myoma uteri with endometrial invasion 2. Endometriosis, polycystic ovarian syndrome 3. BMI > 30 kg/m2 4. No blastocyst available after ovarian stimulation 5. Inadequate cell for laboratory testing
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Apoptotic gene expression Day 5 Blastocyst Threshold cycles from PCR | — |
Secondary
| Measure | Time frame |
|---|---|
| Concordance Rate Day 5 Blastocyst Threshold cycles from PCR between TE biopsy and spent culture media,Apoptotic gene expression between implant and non-implanted embryo within 1 year after testing Threshold cycles from PCR | — |
Countries
Thailand
Contacts
Public ContactPat Leelaamornvichet
King Chulalongkorn Memorial Hospital
Outcome results
None listed