Couples undergoing ICSI treatment Male partner with at least one abnormal semen parameter based on WHO 2021 criteria Microfluidic sperm selection density gradient centrifugation intracytoplasmic sperm injection abnormal semen parameters sibling-oocyte study embryo development
Conditions
Interventions
Sibling metaphase II oocytes allocated to this arm undergo ICSI using sperm prepared by microfluidic sperm selection.,Sibling metaphase II oocytes allocated to this arm undergo ICSI using sperm prepar
Experimental Procedure/Surgery,Active Comparator Procedure/Surgery
Microfluidic sperm selection,Density gradient centrifugation (DGC)
Sponsors
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Eligibility
Sex/Gender
All
Inclusion criteria
Inclusion criteria: Couples undergoing ICSI treatment Male partner with at least one abnormal semen parameter based on WHO 2021 criteria Female age less than 42 years More than 4 dominant follicles on trigger day
Exclusion criteria
Exclusion criteria: Participants with fewer than 4 retrieved oocytes (MII) Sperm volume less than 1.4 ml on the date of oocyte retrieval Pre-preparation TMSC <5 million participants with limited decision-making capacity
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Blastocyst formation Rate day 5-6 after ICSI embryo development assessment by embryologist | — |
Secondary
| Measure | Time frame |
|---|---|
| fertilization rate day 1 after ICSI embryo development assessment by embryologist,Cleavage rate day 3 after ICSI embryo development assessment by embryologist,Euploidy rate after blastocyst biopsy and PGT-A result reporting PGT-A by NGS,good-quality blastocyst rate Day 5-6 after ICSI embryo development assessment by embryologist | — |
Countries
Thailand
Contacts
Public ContactChatsaran Thanapongpibul
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Outcome results
None listed