endothelial keratoplasty ocular surface microbiome, endothelial keratoplasty, long-term topical steroids
Conditions
Interventions
Participants who have endothelial keratoplasty surgery observed from baseline (preoperation) and after 12 months of topical steroids post-surgery,Healthy controls - one-time sampling collection for co
Basic Science,Basic Science
Endothelial keratoplasty (EK) - 28 participants,Healthy control - 10 participants
Sponsors
None listed
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: Participants will be eligible for inclusion if they meet the following criteria: 1. Patients with indications for endothelial keratoplasty (EK) surgery, including EK with or without phacoemulsification. 2. Residents of Thailand. 3. Individuals aged 18 years and older who provide informed consent to participate in the study.
Exclusion criteria
Exclusion criteria: The exclusion criteria will be applied during the screening and enrollment phase to ensure appropriate participant selection: 1. Poorly controlled diabetes mellitus 2. History of graft failure following a previous keratoplasty. 3. History of herpes simplex virus (HSV) keratitis or chronic ocular surface diseases (e.g., blepharitis, conjunctivitis, severe meibomian gland dysfunction, severe dry eye disease ). 4. History of any ocular surgery within the last three months 5. Use of contact lenses, topical antibiotics, or antiglaucoma eye drops within one month prior to sample collection.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Microbiome at baseline (preoperation), 3 months and 12 months after surgery alpha-diversity, beta-diversity, bacterial relative abundance | — |
Secondary
| Measure | Time frame |
|---|---|
| Positive strains, microbial susceptibility results baseline (preoperation), 3 months, 12 months after surgery Conventional culture | — |
Countries
Thailand
Contacts
Public ContactVilavun Puangsricharern
King Chulalongkorn Memorial Hospital
Outcome results
None listed