Effect of No-Rinse Toothbrushing on Salivary Streptococcus mutans, Salivary Fluoride Levels, and Salivary Properties in Healthy Subjects

Status

Recruiting

Phases

Phase 1

Study type

Interventional

Source

TCTR

Registry ID

TCTR20240916011

Enrollment

Registered

2024-09-16

Start date

2024-11-04

Completion date

Unknown

Last updated

2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Healthy volunteers who have at least 20 permanent teeth in the oral cavity, age between 20 and 35, resting salivary flow rate not below 0.3 ml/min. without these conditions including completely loss of natural teeth, allergic to any materials used in the study, history of systemic diseases, took medications that might affect the salivary flow rate and oral bacteria such as anticholinergic drugs, mouthwash, antibiotics within 1 month before taking part in the study, have prosthodontic or orthodon

Interventions

All participants in this group will brush their teeth with the same fluoride toothpaste using the modified Bass technique. Participants will use a pea size amount of toothpaste twice daily for two min

Experimental Procedure/Surgery,Experimental Procedure/Surgery

No rinse toothbrushing,Rinse toothbrushing

Eligibility

Sex/Gender

All

Age

20 Years to 35 Years

Inclusion criteria

Inclusion criteria: a. Healthy men or women who have at least 20 permanent teeth in the oral cavity b. Age between 20 and 35 c. Resting salivary flow rate not below 0.3 ml/min.

Exclusion criteria

Exclusion criteria: a. Complete edentulous (completely loss of natural teeth) b. Allergic to any materials used in the study c. History of systemic diseases d. Took medications that might affect the salivary flow rate and oral bacteria such as anticholinergic drugs, mouthwash, antibiotics within 1 month before taking part in the study e. Subjects who have prosthodontic or orthodontic appliances f. Subjects who have lost more than two teeth in each sextant g. Subjects who have periodontitis h. Infect SAR-CoV-2 or have a positive COVID-19 test within 10 days before the procedure i. History of drinking alcohol or smoking j. Pregnancy k. Unwilling to participate in the study or unable to provide informed consent

Design outcomes

Primary

Measure	Time frame
Salivary s. mutans Baseline (before the intervention), 1 month, and 3 months Measure Salivary s. mutans by CFU counting and DNA levels.	—

Secondary

Measure	Time frame
Salivary fluoride, and salivary properties Baseline (before the intervention), 1 month, and 3 months Measure fluoride in saliva as well as salivary properties including salivary flow rate, pH, and buffering capacity	—

Countries

Thailand

Contacts

Public ContactTipparat Parakaw

Faculty of Dentistry, Mahidol University

tipparat.par@mahidol.ac.th022007833

Outcome results

None listed

Source: TCTR (via WHO ICTRP) · Data processed: Aug 9, 2026