The new anodization modification on titanium interaction with human osteoblasts and fibroblasts. Anodized surface, surface modification, gingival fibroblast, osteoblast, cell adhesion
Conditions
Interventions
machined titanium surfaces ,The titanium discs were sandblasted with aluminum oxide (Al2O3), followed by acid etching using a mixture of 60% sulfuric acids and 30% hydrochloric acid. , anodization pr
control,sandblasted with large grit and acid etch (SLA),anodized A ,anodized B
Sponsors
Faculty of Dentistry
Eligibility
Sex/Gender
All
Age
20 Years to 50 Years
Inclusion criteria
Inclusion criteria: 1. The patient plans to place dental implant 2. Volunteers aged 20-50 years 3. No bone disease 4. No smoking Human gingival fibroblast cells were obtained from the molar teeth of 3 patients, aged between 20-30 years, extracted for orthodontic reasons at the Department of Surgery. No smoking.
Exclusion criteria
Exclusion criteria: 1. Medical health problems 2. Bone disease 3. Smoking
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Osteoblast Cell morphology and Cell adhesion observe at 1 hour and 3 hours scanning electron microscopy and Immunofluorescent staining,Osteoblast Cell proliferation Measurements were taken at 24 hours, 3 days, and 7 days post-seeding. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay (MTT assay),Osteogenically differentiated: Alkaline phosphatase (ALP) activity after 3 and after 7 days post-seeding. Alkaline phosphatase assay (ALP assay),Assessment of mineralization after 14 days and after 21 days post-seeding. Alizarin red S staining | — |
Secondary
| Measure | Time frame |
|---|---|
| Fibroblast Cell morphology and Cell adhesion observe at 1 hour Immunofluorescent staining | — |
Countries
Thailand
Contacts
Public ContactAssist. Prof. Wareeratn Chengprapakorn
Chulalongkorn University
Outcome results
None listed