Dengue infection patients and healthy volunteers who are 18-50 years old. gut leakage microbiome homeostasis systemic immunity dengue
Conditions
Interventions
Participants diagnosed with dengue infection (by dengue antigen NS1 or PCR) with or without warning signs, or severe dengue, and in-hospitalization.,No previously diagnosed dengue fever (by taking his
Diagnostic,Diagnostic
Dengue patient group,Healthy volunteer group
Sponsors
The e-ASIA Joint Research Program; PMU-B
Eligibility
Sex/Gender
All
Age
18 Years to 50 Years
Inclusion criteria
Inclusion criteria: 1. Dengue patient group 1.1 Participants diagnosed with dengue infection (by dengue antigen NS1 or PCR) with or without warning signs, or severe dengue, and in-hospitalization. 1.2 Able to provide written informed consent. 2. Healthy volunteer group 2.1 No previously diagnosed dengue fever (by taking history) within five years. 2.2 Able to provide written informed consent.
Exclusion criteria
Exclusion criteria: 1. Presence of severe comorbidities (e.g. severe pneumonia, co-infection of either local or systemic infections, active cardiovascular or cerebrovascular, active malignancy) at time of admission. 2. Recent use of antibiotic or prebiotic and/or probiotics 3. History of gastrointestinal surgery 4. Pregnancy 5. Non-Thai language speaking participants
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Characterization of fecal microbiota Two separate days during in-hospitalization and one time-point collection for healthy volunteers High-throughput DNA sequencing | — |
Secondary
| Measure | Time frame |
|---|---|
| Exosome identification At the enrollment and another collected on day 7 of onset RT-qPCR for microRNAs,Gut permeability At the enrollment and another collected on day 7 of onset Serum concentration of human zonolin; serum calprotectin; serum beta-D-glucan; serum occludin; serum TJP1; serum lipopolysaccharide,Cell-free bacterial DNA At the enrollment and another collected on day 7 of onset Fluorescence-based assay,Neutrophil extracellular traps and low-density granulocytes At the enrollment and another collected on day 7 of onset Isolation PBMC and PMN for NETs and LDGs detection | — |
Countries
Thailand
Contacts
Public ContactWiwat Chancharoenthana
Mahidol University
Outcome results
None listed