Women diagnosed with infertility underwent treatment with an assisted reproductive technology procedure, specifically in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) with the indications for preimplantation genetic screening to screen for genetic abnormalities before the embryos were transferred day 5 blastocyst, day 6 blastocyst, transcriptomics, gene expression, implantation
Conditions
Interventions
Blastocyst which developed to day 5 of life with normal chromosome result from PGT-A ,Blastocyst which developed to day 6 of life with normal chromosome result from PGT-A
Basic Science,Basic Science
Euploid blastocyst day 5 of developement,Euploid blastocyst day 6 of development
Sponsors
Ratchadapisek Sompoch Endowment Fund
Eligibility
Sex/Gender
Female
Age
18 Years to 42 Years
Inclusion criteria
Inclusion criteria: 1. Women diagnosed with infertility underwent treatment using IVF or ICSI with indication to test for preimplantation genetic testing for aneuploidy (PGT-A) to screen for genetic abnormalities before the embryos were transferred. 2. The women received ovarian stimulation using the GnRH antagonist protocol, which involved the administration of recombinant gonadotropins for ovarian stimulation and the use of a GnRH antagonist to suppress premature ovulation. They also received human chorionic gonadotropin or a GnRH agonist to trigger ovulation. 3. The women's age ranged from 18 to 42 years old. 4. The embryos reached the blastocyst stage and underwent PGT-A, which confirmed that they had a normal chromosomal count (euploidy: 46XX or 46XY).
Exclusion criteria
Exclusion criteria: 1. Women with abnormalities in the uterine cavity, such as endometrial polyps or myomas with endometrial invasion, may experience impaired fertility and affect the pregnancy rate. 2. Women diagnosed with endometriosis or polycystic ovarian syndrome (PCOS). 3. Body mass index (BMI) equal to or greater than 30 kg/m2
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| difference of gene expression immediate after recruitment RT-qPCR | — |
Secondary
| Measure | Time frame |
|---|---|
| Implantation rate 2 weeks after embryo transfer beta-hcg,Pregnancy rate 6 weeks after embryo transfer ultrasound ,miscarriage rate after event occurred review medical record | — |
Countries
Thailand
Contacts
Public ContactChularat Suwandee
Faculty of Medicine, Chulalongkorn University
Outcome results
None listed