Sleep Deprivation Sleep Deprivation, L-Arginine, Anaerobic Exercise, Apoptosis, BMAL1, CCAR2
Conditions
Interventions
Participants included 20 healthy men, randomized into the L-arginine intervention group and a placebo control. Subjects in the intervention group took one 1000 mg L-arginine tablet daily for 8 weeks w
clock and wake up between 7 and 8 o'
clock the following day. Sleep deprivation was enforced for 24 hours from 8 a.m. to 8 a.m (The participants were asked to stop their nocturnal and avoided any daytime napping). the next day by reading
Placebo Comparator Drug,Experimental Behavioral,Experimental Other
L arginine supplementation ,Sleep deprivation ,Anaerobic exercise
Sponsors
Baqiyatallah University of Medical Sciences
Eligibility
Sex/Gender
Male
Age
26 Years to 35 Years
Inclusion criteria
Inclusion criteria: 1. Having physical health 2. Having mental health and having good sleep quality 3. Having normal sleep of about 8 hours a day 4. Not using any tobacco products 5. Having an age range of 25 to 35 years 6. Having a body mass index less than 28.
Exclusion criteria
Exclusion criteria: 1. Participating in heavy activities during research 2. Use of any tobacco substance during research 3. Getting any disease during research 4. Suffering severe physical injury during the research 5. Taking any kind of medicine during research.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Bax Gene expression The first blood sample was taken after 8 hours of normal sleep (time 1), then subjects performed anaerobic exercise and immediately post-exercise a second blood sample was taken (time 2). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs).,Bcl2 gene expression The first blood sample was taken after 8 hours of normal sleep (time 1), then subjects performed anaerobic exercise and immediately post-exercise a second blood sample was taken (time 2). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs).,BMAL1 gene expression The first blood sample was taken after 8 hours of normal sleep (time 1), then subjects performed anaerobic exercise and immediately post-exercise a second blood sample was taken (time 2). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs).,CCAR2 The first blood sample was taken after 8 hours of normal sleep (time 1), then subjects performed anaerobic exercise and immediately post-exercise a second blood sample was taken (time 2). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs). | — |
Secondary
| Measure | Time frame |
|---|---|
| Bcl2 gene expression after dietary supplement for 8 weeks, a third sample was taken (time 3),after sleep deprivation the fourth sampling was taken (time 4). anaerobic exercise was repeated and a sample was taken (time 5). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs).,Bax gene expression after dietary supplement for 8 weeks, a third sample was taken (time 3),after sleep deprivation the fourth sampling was taken (time 4). anaerobic exercise was repeated and a sample was taken (time 5). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs).,BMAL1 gene expression after dietary supplement for 8 weeks, a third sample was taken (time 3),after sleep deprivation the fourth sampling was taken (time 4). anaerobic exercise was repeated and a sample was taken (time 5). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs).,CCAR2 after dietary supplement for 8 weeks, a third sample was taken (time 3),after sleep deprivation the fourth sampling was taken (time 4). anaerobic exercise was repeated and a sample was taken (time 5). The RT-Pcr method was used to determine apoptosis gene expression in peripheral blood mononuclear cells (PBMCs). | — |
Countries
Iran
Contacts
Public ContactMirzahossein Norouzi kamareh
Baqiyatallah University of Medical Sciences
Outcome results
None listed