DNA damage following isoflurane infusion intraoperatively Dexmedetomidine, Isoflurane oxidative stress DNA damage
Conditions
Interventions
The group A patients received anesthetic drugs according to the established protocol of general anesthesia and maintained with ISO at nearly Minimum alveolar concentration of 1.2 MAC.Intravenous blood
Experimental Drug,Experimental Drug
Group A (ISO alone) (n-12),Group B (ISO and DEX) (n-12)
Sponsors
University of veterinary and animals science Lahore, Pakistan
Eligibility
Sex/Gender
Male
Age
18 Years to 50 Years
Inclusion criteria
Inclusion criteria: Male patients aged from 18 to 50 years, will be classified by American Society of Anesthesiology (ASA) with physical status of 1-II (including healthy patients with no existing systemic disease other than surgical abnormality) undergoing invasive emergency surgeries lasting for at least four hours will be included in the study.
Exclusion criteria
Exclusion criteria: ASA (American society of Anesthesiology) class 3 and 4, Diabetics, Overweight individuals, those with recently intake of antioxidant supplements or any medication, those who received radiation recently and elderly patients with decreased intravascular volume as they are prone to hypotension, will be excluded from the study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| DNA Damage 0, 4, 8, and 16 hours of anaesthesia induction Single Cell Gel Electrophoresis (SCGE)/Comet Assay | — |
Secondary
| Measure | Time frame |
|---|---|
| oxidative stress marker malondialdehyde (MDA) ,endogenous antioxidants superoxide dismutases (SOD) and catalases (CAT). 0, 4, 8, and 16 hours of anaesthesia induction (Ohkawa et al.) for lipid peroxides . 1979; (Kakkar et al. )spectrophotometric assay of superoxide dismutase. 1984. (Hadwan et al.) spectrophotometric method for catalase activity. | — |
Countries
Pakistan
Contacts
Public Contactsadaf aroosa
university of veterinary and animal science Lahore Pakistan
Outcome results
None listed