Cytotoxic and genotoxic effect of buccal mucosal cells in patients wearing fixed-orthodontic appliances. Cytotoxicity Genotoxicity Fluoride Orthodontic appliances
Conditions
Interventions
The subjects were bonded with fixed orthodontic appliances on both arches and did not receive professional fluoride application. ,The subjects were bonded with fixed orthodontic appliances on both arc
No Intervention No treatment,Experimental Drug,Experimental Drug,Experimental Drug
Control group,Acidulated phosphate fluoride gel ,Neutral fluoride gel ,Fluoride varnish
Sponsors
Faculty of Dentistry, Chulalongkorn University, Thailand (Lecturer Fund)
Eligibility
Sex/Gender
All
Age
No minimum to 35 Years
Inclusion criteria
Inclusion criteria: 1.permanent dentition with all second molars erupted in the oral cavity 2.clinically healthy oral mucosa 3.no previous orthodontic treatment 4.no occupational exposure to metals 5.no amalgam fillings or metal restorations 6.no lingual appliances or extraoral auxiliary appliances
Exclusion criteria
Exclusion criteria: 1.smoking 2.pre-existing systemic diseases or medication associated with oral mucosa changes
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Level of metal ions in buccal mucosal cells Before treatment and 3 months after appliance placement Inductively coupled plasma-mass spectrometry (ICP-MS) | — |
Secondary
| Measure | Time frame |
|---|---|
| Cell viability Before treatment and 3 months after appliance placement Trypan blue exclusion test ,Frequency of cellular death Before treatment and 3 months after appliance placement The cells were underwent Papanicolaou staining and were scored using a light microscope.,Number of micronucleated cell Before treatment and 3 months after appliance placement The cells were underwent Papanicolaou staining and were scored using a light microscope. | — |
Countries
Thailand
Contacts
Public ContactPintuon Chantarawaratit
Department of Orthodontics, Faculty of Dentistry, Chulalongkorn University
Outcome results
None listed