Type 2 Diabetes mellitus Ocular surface microbiome Conjunctival microbiome Human microbiome Diabetes mellitus DM Diabetes retinopathy DR Next generation sequencing NGS
Conditions
Interventions
Subjects who have diagnosed with type 2 diabetes mellitus according to the
ADA (American Diabetes Association) diagnostic criteria or the subjects with a known
history of diabetes mellitus which the p
Basic Science,Basic Science
Diabetes group,Non-diabetes group
Sponsors
Ratchadapisek Sompoch fund
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: 1. Subjects aged over 18 years old 2. In diabetes group: subjects who have diagnosed with type 2 diabetes mellitus according to the ADA (American Diabetes Association) diagnostic criteria or the subjects with a known history of diabetes mellitus which the patients were under the care of physicians 3. In control group: subjects who have no diabetes mellitus and history of normal blood sugar or HbA1c screening for diabetes within 1 year.
Exclusion criteria
Exclusion criteria: 1. Ocular surface diseases (ex. significant dry eye, Meibomian gland dysfunction, Stevens-Johnson syndrome) 2. History of ocular and periocular infection/inflammation, allergic conjunctivitis within 3 months 3. History of Previous contact lens used within 3 months 4. History ocular medication used (except for non-preservative artificial tear) within 1 month (such as topical antibiotic, topical steroid, intravitreal drug injection etc.) 5. History of ocular surgery within past 3 months 6. History of systemic antibiotic, steroid or immunosuppressive drug used within past 3 months 7. Health care workers
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Ocular surface microbiome in diabetes mellitus patients, compared with control at the time of specimen collection Bioinformatic data : relative abundance, alpha/beta diversity | — |
Secondary
| Measure | Time frame |
|---|---|
| Ocular surface microbiome in the different stage of diabetes retinopathy at the time of specimen collection Bioinformatic data : relative abundance, alpha/beta diversity,Ocular surface microbiome in diabetes mellitus according to HbA1C level at the time of specimen collection Bioinformatic data : relative abundance, alpha/beta diversity,Culture positive and antibiotic resistance at the time of specimen collection Percentage of culture positive and antibiotic resistance | — |
Countries
Thailand
Contacts
Public ContactOrathai Suwajanakorn
Chulalongkorn university
Outcome results
None listed