Dry eye disease Concentration of epitheliotrophic growth factors in PRP and AS Efficacy in treatment of DED of PRP and AS Stability of epitheliotrophic growth factors in PRP and AS epitheliotrophic growth factor autologous serum platelet-
Conditions
Interventions
• The whole blood,
collected from the patients,
will be prepared into a sterile tube without any anticoagulant added.
• The autologous blood was incubated at 31 C° for 2 hours,
and then was centrifuged 3000×g for 30 minutes at 4 C°.
• The supernatant was then collected,
sterilized and aliquoted.
Participants are instructed to instill AS and PRP to each eye separately.,• The whole blood,
will be prepared into a sterile tube with 5 ml of 3.8% sodium citrate.
• The autologous blood was immediately centrifuged 2,
200×g for 10 minutes at 22 ± 2°C.
• The platelet-
rich plasma was collected,
sterilized and aliquoted.
Participants are instructed to instill AS and PRP to each eye separately.
Autologous serum(AS),Platelet-
rich plasma(PRP)
Sponsors
Siriraj Research Fund
Eligibility
Sex/Gender
All
Age
18 Years to No maximum
Inclusion criteria
Inclusion criteria: The patients will be included to the study, when they meet all of the criteria Dry Eye Disease Diagnostic Test Battery: (modified from TFOS DEWS II; Diagnostic Methodology report) OSDI (Ocular Surface Disease Index) ≥ 13 Diagnostic Testing (Homeostasis Markers) Fluorescein Breakup Time 5 corneal spots or - > 9 conjunctival spots or - Lid margin (≥ 2mm length & ≥ 25% width)
Exclusion criteria
Exclusion criteria: Taking immunosuppressive drugs AIDs patients or other immunodeficiency conditions Having serious systemic underlying diseases Pregnant women Unequal severity of dry eye disease between two eyes
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Concentration of epitheliotrophic growth factors in Platelet-Rich Plasma and Autologous Serum At the time of intervention Enzyme-linked immunosorbent assay (ELISA) according to the manufacturers’ instruction | — |
Secondary
| Measure | Time frame |
|---|---|
| The efficacy of PRP and AS in DED 1 month Dry eye ocular symptoms (OSDI score and Dry Eye Questionnaire, used in The Osaka Study16) and signs(,the stability of epitheliotrophic growth factors in PRP and AS Baselline, 1 month, 6 months enzyme-linked immunosorbent assay (ELISA) according to the manufacturers’ instruction at 1 week, 1 | — |
Contacts
Public ContactPanotsom Ngowyutagon
Faculty of Medicine Siriraj Hospital, Mahidol University
Outcome results
None listed