Type two diabetes and non type two diabetes mellitus
Conditions
Interventions
labelled S. aureus (MOI30) at 15,
30 and 60 min. after that,
3 ug/ml hydroethidine (HE) will be added for 5 min and lysed RBC for 10 min. 1xPBS and 3 ug/ml PMA will be used as negative and positive control respectively.
3) miRNA measurement
To determine micro
microRNA microarray will be performed to get significantly expressed microRNAs and then validated by qRT-
PCR.
4) Telomere length
Leukocyte telomere length will be measured by using Cawthon’s method based on polymerase chain reaction technique. The ratio of telomere repeat copy number (T) and the sing
DM group,
1) Vitamin C co-
Type 2 diabetes mellitus group
1) Vitamin C co-
culture
Whole blood from the participants will be collected in heparinize tube. Then,
the blood will be co-
culture with or without vitamin C for 1 hour at 37 °C,
5% CO2.
2) PMN function
After co-
culture,
the sample will be investigated PMN function by measurement %phagocytosis and oxidative burst using flow cytometry. In brief,
100 ul of blood will be mixed with 100 ul of FITC-
Sponsors
Khon Kaen University
The Royal Golden Jubilee (RGJ) Ph.D. Programme The Thailand Research Fund (TRF)
Eligibility
Sex/Gender
All
Age
30 Years to 60 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria of healthy control include age between 30 and 60 years old, Non type two diabetes mellitus, normal resting EKG, lack of any chronic diseases and chronic infections such as HIV, hepatitis and TB, have no consumption any supplementary at least 3 months, lack of any regular exercise program for at least 6 months and live in Khon Kaen Province, Thailand. For T2DM, the inclusion criteria include age between 30 and 60 years old, being diagnosed as type 2 diabetes mellitus for at least 12 months, being treated with oral diabetic drugs only (no insulin injection), receiving lipid-lowing drugs or anti-hypertensive drugs, sedentary and lack of any regular exercise program for at least 6 months and living in Khon Kaen Province, Thailand.
Exclusion criteria
Exclusion criteria: Abnormal resting EKG, chronic diseases and chronic infections such as HIV, hepatitis and TB, have consumption any supplementary at least 3 months, have a regular exercise program for at least 6 months. For T2DM, being diagnosed as type 2 diabetes mellitus early than 12 months, being treated with insulin injection.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Telomere length 1 day Real time PCR based technique,immune response 1 day Flow cytometry,microRNA expression 1 day microarray,microRNA expression 1 day microarray | — |
Secondary
| Measure | Time frame |
|---|---|
| Heart rate availability 5 minutes Detected by SA-3000P | — |
Countries
Thailand
Contacts
Public ContactRujira Nonsa-ard
ฺGraduate school (Biomedical Science program), Khon Kaen University
Outcome results
None listed