LINE-
Conditions
Interventions
Dental pulp stem cells which extract from impacted tooth were cultured 14 days as control group.
,Osteoblasts which differentiated from dental pulp stem cells. Cultured in osteogenic medium for 14 day
Basic Science,Basic Science
Dental pulp stem cells ,Dental pulp stem cell derived osteoblasts
Sponsors
Chulalongkorn University
Eligibility
Sex/Gender
All
Age
20 Years to 25 Years
Inclusion criteria
Inclusion criteria: The non-pathological third molars removed from healthy adult subjects (N=13) who seek treatment for impacted third molars at the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chulalongkorn University will be used. After the consents for this study are signed, the dental pulp tissue will then be isolated in sterile condition
Exclusion criteria
Exclusion criteria: 1.Patient whose third molars have pathology 2.Patient whose third molars are needed to odontectomy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| LINE-1, Alu and HERV-K methylation level 14 days after differentiated dental pulp stem cell to osteoblast quantitative combined bisulfite restriction analysis(qCOBRA) technique | — |
Secondary
| Measure | Time frame |
|---|---|
| LINE-1, Alu and HERV-K methylation pattern 14 days after differentiated dental pulp stem cell to osteoblast quantitative combined bisulfite restriction analysis(qCOBRA) technique | — |
Countries
Thailand
Contacts
Public ContactKeskanya Subbalekha
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chulalongkorn University
Outcome results
None listed