Limbal stem cell deficiency after COMET Limbal stem cell deficiency LSCD cultivated oral mucosal epithelial transplantation COMET Limbal stem cells Stem cells Cornea
Conditions
Interventions
Impression cytology and confocal microscopy were done in each patient who underwent COMET for at least 3 years to characterize the corneal surface after COMET whether it consists of corneal,
conjunctival or oral mucosal epithelial cells,
and corneal buttons from some patient who underwent penetrating keratoplasty after COMET were analyzed by immunohistochemistry and compared to impression cytology,
vivo confocal microscopy and clinical outcome.
Experimental Procedure/Surgery
Sponsors
Faculty of Medicine Siriraj Hospital, Mahidol University
Department of Ophthalmology,
Faculty of Medicine Siriraj Hospital,
Mahidol University
Eligibility
Sex/Gender
All
Age
18 Years to 0 Years
Inclusion criteria
Inclusion criteria: 1. Patient who diagnosed with unilateral or bilateral limbal stem cell deficiency from several causes eg. Stevens-Johnson syndrome, Toxic epidermal necrolysis, Chemical burn, chronic inflammation, multiple surgery, Long term topical drugs usage or idiopathic. 2. Patient who underwent cultivated oral mucosal epithelial transplantation (COMET) at least for 3 years, including patient who underwent Penetrating keratoplasty after COMET 3. Patients with total or partial limbal stem cell deficiency in the eye that underwent COMET 4. Preoperative impression cytology results showed that the patient had limbal stem cell deficiency
Exclusion criteria
Exclusion criteria: 1. Patient age under 18 years old 2. Patient who had HIV infection 3. Patient who was diagnosed with active ocular infection 4. Patient with uncontrolled glaucoma 5. Pregnant patient 6. Patient who not willing to follow up 7. Patient who are not cooperate in treatment, either from psychological or physical problems
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Phenotypic characteristics of impression cytology of cornea after COMET On the latest follow up visit Impression cytology and immunofluorescence for specific markers | — |
Secondary
| Measure | Time frame |
|---|---|
| Correlation of impression cytology with in vivo confocal microscopy and clinical outcome On the latest follow up visit Impression cytology, slit-lamp examination and in vivo confocal microscopy,Correlation of impression cytology with immunohistochemistry study from corneal button On the latest follow up visit Corneal buttons from patients who underwent penetrating keratoplasty were analyzed by pathologist | — |
Contacts
Public ContactPinnita Prabhasawat
Department of Ophthalmology, Faculty of Medicine Siriraj Hospital, Mahidol University
Outcome results
None listed