11 patients with normal renal function who underwent abdominal surgery and were excluded from chronic disease were recruitedas control groups.
Conditions
Interventions
In Nephropathy center of the First Affiliated Hospital of Xi’an Jiaotong University,
34 uremic patients were recruited from August to December 2013. Inclusion criteria: 1. Laboratory diagnosised end-
stage renal disease. 2. undergoing catherization for peritoneal dialysis. Exclusion criteria: patients with chronic diseases such as chronic hepatitis,
skin disease and systemic lupus erythematosus were excluded. 11 patients with normal renal function who underwent abdominal surgery and were excluded from chronic disease were recruitedas control grou
0.999× Age-
0.176×BUN (mg/dL)-
0.170×Alb (mg/dL)+0.318 (0.762 for female). For all patients,
0.2 cm×0.5 cm×1.0 cm skin biopsies were taken from the lower abdomen (4 cm above the pubic symphysis). Written informed consent was obtained from every participant. In our experiment,
the positive control of UTs were renal medullary tissues of patients with early stage of chronic kidney disease suffering renal biopsy (3 cases). For Immunohistochemistry,
the positive control of AQP5 was apocrine sweat gland of osmidrosis patients from plastic surgery underwent apocrine sweat gland removal (5 cases). Both with PBS instead of primary antibody as negativ
all specimens were divided into 3 parts,
one fixed in 4% paraformaldehyde,
fixed about 12-
then embedded in paraffin,
sliced. the other two eye cutting and then separated into a sterile enzyme free freezing tube,
immediatel
Treatment
uremic patients
Sponsors
Jing Lv
Liyi Xie
Eligibility
Sex/Gender
All
Age
38 Years to 67 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria: 1. Laboratory diagnosised end-stage renal disease. 2. undergoing catherization for peritoneal dialysis.
Exclusion criteria
Exclusion criteria: Exclusion criteria: patients with chronic diseases such as chronic hepatitis, skin disease and systemic lupus erythematosus were excluded.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The abundance of UT-A1 and UT-B1 in uremic sweat glands is significantly increased in uremic patient 01/12/2016 Immunohistochemistry, immunofluorescence, RT-PCR and Western Blot | — |
Secondary
| Measure | Time frame |
|---|---|
| The expression of AQP5 is significantly decreased in uremic patients. 01/12/2016 Immunohistochemistry, immunofluorescence, RT-PCR and Western Blot | — |
Countries
China
Contacts
Public ContactJing Lv
xi'an jiaotong university
Outcome results
None listed