The Improvement of ovarian cortex survival by supplementation of Growth Hormone (GH) in freezing media.

The improvement of ovarian cortex survival by supplementation of Growth Hormone (GH) in freezing media.

Status

Recruiting

Phases

Phase 1

Study type

Interventional

Source

TCTR

Registry ID

TCTR20170324002

Enrollment

Unknown

Registered

2017-03-24

Start date

2017-03-17

Completion date

Unknown

Last updated

2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

The effect of growth hormone added in vitrification solution for preserve the human ovarian tissue. growth hormone&#44

Interventions

Fresh tissue,Vitrification without growth hormone,Vitrification with Growth hormone

The ovarian tissue was fixed immediately with 10% neutral buffered formalin / 4% paraformaldehyde and used for fresh histological analysis(wouldn&#39

t undergone vitrified cryopreservation).,Ovarian tissue would undergone vitrified cryopreservation&#44

which all three incubation steps were not consisted of GHH in vitrified solutions.,Supplemented with 50 ng/ ml of GH(Eutropin&#44

LG) in the vitrified solution.

No Intervention No treatment,Placebo Comparator No treatment,Placebo Comparator Drug

Eligibility

Sex/Gender

Female

Age

20 Years to 40 Years

Inclusion criteria

Inclusion criteria: 1 The patient has a benign ovarian cyst confirmed with ultrasound and the treatment plan is to undergo a unilateral oophorectomy or bilateral oophorectomy by laparotomy or laparoscopy between 25 Januaray to 30 March 2017 2 Informed consent by the patient

Exclusion criteria

Exclusion criteria: 1 During the operation the cyst looked malignant or suggested malignant from frozen section 2 The surgeon considered that for the benefit of the patient the surgery should be converted from oophorectomy to cystectomy only 3 The ovarian cortex retrieved is not appropriate for the size of 2 x 2 x 0 1 cm which may be caused by the size of the cyst

Design outcomes

Primary

Measure	Time frame
Percentage of intact follicles following ovarian tissue vitrification . 7-14 days after freezing H&E with light microscopy.	—

Secondary

Measure	Time frame
Percentage of intact follicles following ovarian tissue vitrification . 7-14 days after freezing Transmission electron microscopy.	—

Countries

Thailand

Contacts

Public ContactKringsak Dhanaworavibul

The Department of Obstetrics and Gynecology, Factory of Medicine, Prince of Songkla University

dkrieng@yahoo.com+6681-5998-598

Outcome results

None listed

Source: TCTR (via WHO ICTRP) · Data processed: Aug 9, 2026