The effect of FSH added in vitrification solusion for preserve the human ovarian tissue. Ovarian tissue cryopreservation,
Conditions
Interventions
Supplemented with 0.3 IU/ ml of FSH (Gonal-
f,
Merch,
Germany) in the vitrified solutions.,Ovarian tissue would undergone vitrified cryopreservation,
which all three incubation steps weren’t consisted of FSH in vitrified solutions.,The ovarian tissue was fixed immediately with 10% neutral buffered formalin / 4% paraformaldehyde and used for fresh
t undergone vitrified cryopreservation).
Placebo Comparator Drug,Placebo Comparator No treatment,No Intervention No treatment
Follicle-
Stimulating Hormone (FSH),placebo,controlled
Sponsors
The Factory of Medicine, Prince of Songkla University
Eligibility
Sex/Gender
Female
Age
20 Years to 40 Years
Inclusion criteria
Inclusion criteria: 1.The patient has a benign ovarian cyst confirmed with ultrasound and the treatment plan is to undergo a unilateral oophorectomy or bilateral oophorectomy by laparotomy or laparoscopy between 25 Januaray to 30 March 2017. 2.Informed consent by the patient.
Exclusion criteria
Exclusion criteria: 1.During the operation, the cyst looked malignant or suggested malignant from frozen section. 2.The surgeon considered that for the benefit of the patient the surgery should be converted from oophorectomy to cystectomy only. 3.The ovarian cortex retrieved is not appropriate for the size of 2 x 2 x 0.1 cm., which may be caused by the size of the cyst.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Percentage of intact follicles following ovarian tissue vitrification . 24 hours after tissue culture H&E with light microscopy. | — |
Secondary
| Measure | Time frame |
|---|---|
| Percentage of intact follicles following ovarian tissue vitrification . 24 hours after tissue culture Transmission electron microscopy | — |
Countries
Thailand
Contacts
Public ContactKringsak Dhanaworavibul
+6674-285-349
Outcome results
None listed