Phenotypic characterization by impression cytology of the corneal surface reconstructed by simple limbal epithelial transplantation

Phenotypic characterization by impression cytology of the corneal surface reconstructed by simple limbal epithelial transplantation (SLET)

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

TCTR

Registry ID

TCTR20160310002

Enrollment

Registered

2016-03-10

Start date

2016-02-01

Completion date

Unknown

Last updated

2026-03-30

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Limbal stem cell deficiency after SLET Limbal stem cell deficiency LSCD Simple limbal epithelium transplantation SLET Limbal stem cells Stem cells Cornea

Interventions

Impression cytology and confocal microscopy are performed 3 times&#44

3 months apart to characterize the corneal surface after SLET whether it consists of corneal or conjunctival epithelial cells.

Experimental Procedure/Surgery

Impression cytology and in vivo confocal microscopy

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Inclusion criteria

Inclusion criteria: 1. Patients with unilateral or bilateral limbal stem cell deficiency (LSCD) from various causes including Stevens-Johnson syndrome, chemical or thermal burns, chronic keratitis, multiple corneal surgeries, and prolonged use of eye drops 2. Patients having experienced auto-SLET or allo-SLET 3. Patients who used to have been diagnosed total LSCD in the SLET-treated eye 4. Patients who have been diagnosed LSCD with impression cytology before receiving SLET

Exclusion criteria

Exclusion criteria: 1.Patients aged below 18 years 2. Patients who used to have been diagnosed partial LSCD in the SLET-treated eye 3. Patients having received penetrating keratoplasty following SLET 4. HIV-infected patients 5. Patients with ongoing eye infection 6. Patients with uncontrolled glaucoma 7. Patients with pregnancy 8. Patients unable to follow-up 9. Patients with non-compliance, physically or mentally

Design outcomes

Primary

Measure	Time frame
Characteristics of impression cytology of cornea after SLET Three time points of 3-month interval, all post-operative Impression cytology and immunofluorescence for specific markers of corneal and conjunctival epitheli	—

Secondary

Measure	Time frame
Correlation of impression cytology with in vivo confocal microscopy and clinical outcome Three time points of 3-month intervall, all post-operative Impression cytology, slit-lamp examination and in vivo confocal microscopy	—

Contacts

Public ContactPinnita Prabhasawat

Unaffiliated

pinnita.pra@mahidol.ac.th02-4196635

Outcome results

None listed

Source: TCTR (via WHO ICTRP) · Data processed: Apr 4, 2026