Dental caries Probiotics Lactobacillus casei Shirota Yakult Dental caries Dental caries susceptibility Dental caries activity tests Acidogenicity Stephan curve
Conditions
Interventions
All participants received 7 x 100 ml bottles of standard Yakult at the start of the study. Participants were instructed to consume one bottle daily for 7 consecutive days,
after dinner and over a period of 30 seconds.
Experimental Dietary Supplement
Sponsors
Faculty of Dentistry Undergraduate Research Opportunities Programme
Eligibility
Sex/Gender
All
Age
21 Years to 40 Years
Inclusion criteria
Inclusion criteria: Healthy (no systemic disease)
Exclusion criteria
Exclusion criteria: Smoking Alcoholism Pregnancy Prior dental restorations on the maxillary canines and premolars Habitual consumers of Yakult® probiotic beverage
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Area under the Stephan curve Before intervention (day 1); 7 and 21 days after experiment start Area under Stephan curve (pH/time) bounded by curve and pH 5.5, after 10% sucrose challenge.,Lowest pH Before intervention (day 1); 7 and 21 days after experiment start Minimum pH observed from the Stephan curve,pH recovery time Before intervention (day 1); 7 and 21 days after experiment start Duration between the two times the Stephan curve touched the horizontal line denoting pH 5.5 | — |
Secondary
| Measure | Time frame |
|---|---|
| Levels of plaque Streptococcus mutans and Lactobacillus casei Shirota bacteria Before intervention (day 1); 7 and 21 days after experiment start Real-time PCR specific for S. mutans and L. casei strain Shirota bacteria,Cariogram risk assessment Experiment start (day 1) Survey; mutans streptococci and Lactobacillus counts; salivary buffer capacity | — |
Countries
Singapore
Contacts
Public ContactChin-Ying Hsu
Faculty of Dentistry, National University of Singapore
Outcome results
None listed