Metabolic syndrome body composition cardiorespiratory fitness inflammatory markers sports exercise health
Conditions
Interventions
Sponsors
Eligibility
Inclusion criteria
Inclusion criteria: Healthy volunteers; both sexes; non smokers; age between 12 and 17 years; BMI above 2 standard deviations of references values established by the WHO for sex and age; Tanner maturation stages based on pubic hair between 4 and 5.
Exclusion criteria
Exclusion criteria: Medical conditions contraindicating the participation in physical activities; use of medication influencing metabolic or cardiovascular function; participation in weight management programs within 6 months prior to the experiment.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Anthropometric measurements (body mass, height, hip circumference, waist circumference) 12 weeks after the beginning of intervention Digital scale, fixed stadiometer, metal anthropometric tape.,Blood pressure at rest 12 weeks after the beginning of intervention automated Omron 705IT device,Cardiorespiratory fitness 12 weeks after the beginning of intervention incremental cardiopulmonary stress test performed on a cycle ergometer,Lipid blood profile (tryglicerides, colestherol, LDL, HDL 12 weeks after the beginning of intervention Determined fluorometrically using an automated analyzer and enzymatic kits,Insulin levels and C-reactive protein 12 weeks after the beginning of intervention enzyme immunoassay ELISA kit and highly sensitive latex with an automatic analyzer,Oral glucose tolerance and HOMA-IR 12 weeks after the beginning of intervention blood sample after 12h fasting followed by ingestion of 75g of dextrose,endothelial function 12 weeks after the beginning of intervention enous occlusion plethysmography,Adiponectin, interleukin-6 (IL-6), leptin, tumor necrosis factor-alpha (TNF-alfa) 12 weeks after the beginning of intervention ELISA (Enzyme Linked Immunosorbent Assay),non-esterified fatty acids (NEFA) 12 weeks after the beginning of intervention enzymatic colorimetric assay method | — |
Secondary
| Measure | Time frame |
|---|---|
| biological maturity stage 12 weeks after the beginning of intervention maturation of pubic hair evaluated in pediatric clinical examination,heart rate variability 12 weeks after the beginning of intervention heart rate monitor (Polar RS800CX, PolarTM, Kempele, Finland) | — |
Countries
Portugal
Contacts
University of Porto