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Comparative Methods in Identifying Causative Microorganisms in Presumed Bacterial Endophthalmitis: Blood Culture System, PCR, and Conventional System

Comparative Methods in Identifying Causative Bacterial Microorganisms in Presumed Acute Endophthalmitis: Conventional method, Blood Culture System, and PCR technique

Status
Active, not recruiting
Phases
Unknown
Study type
Observational
Source
TCTR
Registry ID
TCTR20110000024
Enrollment
Unknown
Registered
2012-03-30
Start date
2012-03-02
Completion date
Unknown
Last updated
2026-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

yield of culture positive by 3 methods&#44

Interventions

Vitreous specimens were underwent microorganism identification by conventional culture technique.,Vitreous specimens were underwent microorganism identification by PCR technique.,Vitreous specimens we
Diagnostic,Diagnostic,Diagnostic
Conventional culture,PCR,Blood culture system

Sponsors

Ratchadaphiseksomphot Endowment Fund
Lead Sponsor

Eligibility

Sex/Gender
All
Age
18 Years to No maximum

Inclusion criteria

Inclusion criteria: All cases of presumed bacterial endophthalmitis which are defined as the patients with 1. Best corrected visual acuity 20/50 or worse 2. Symptoms and signs of bacterial endophthalmitis no longer than 3 weeks: sudden visual loss, ocular pain, photophobia, red eye, A/C flare and cell, hypopyon and vitreous cell or clumping 3. In case of clinical suspect of bacterial endophthalmitis but posterior segment cannot be visualized. There must be positive finding of endophthalmitis from B-scan ultrasound 4. Etiology from recent post intraocular surgery, intraocular trauma or endogeneous infection.

Exclusion criteria

Exclusion criteria: All patients with 1. Suspected non-bacterial endophthalmitis 2. History of uveitis 3. Inadequate specimen for laboratory analysis 4. The patient is not performed vitreous tapping or PPV or evisceration in King Chulalongkorn memorial hospital

Design outcomes

Primary

MeasureTime frame
To compare the yield of detecting and identifying the causative organisms of presumed bacterial endo within 1-2 weeks after specimen collection the yield of culture positive micro organism in these 3 methods

Secondary

MeasureTime frame
1. To identify an incidence of presumed bacterial endophthalmitis and cultured positive endophthalmi an incidence of culture positive endhophthalmitis cases

Countries

Thailand

Contacts

Public ContactPear Pongsachareonnont

Chulalongkorn Memorial Hospital

Outcome results

None listed

Source: TCTR (via WHO ICTRP) · Data processed: Aug 9, 2026