Dengue
Conditions
Interventions
The study will be conducted on Professorial Paediatric Unit (giving vitamin E) and Ward 4 (control). Both are General Paediatric medical ward at the Lady Ridgway Hospital for Children Colombo (LRH).
A
Sponsors
Professor Sharmila Jayasena
Eligibility
Inclusion criteria
Inclusion criteria: Children between the ages of 5–14 years within first 84 hours of onset of fever, in whom dengue infection is clinically suspected or immunologically diagnosed (NS1 positive). Clinical suspicion is made based on along with 2 of the other clinical symptoms: headache, retro-orbital pain, myalgia, arthralgia, bone pain, leucopaenia (WBC count 10% above baseline) as described by the national guidelines published by Ministry of Health Sri Lanka
Exclusion criteria
Exclusion criteria: Any child on long-term medication, underlying chronic illness such as cardiac, renal or hepatic disease and those taking vitamin supplements.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Serum Total Antioxidant Capacity - Measured using the ABTS assay. The ABTS assay is a colorimetric assay that measures the antioxidant capacity of a substance. The antioxidant levels will be determined using the calibration curve of Trolox and results are expressed as Trolox Equivalent Antioxidant Capacity (TEAC). [Serum samples will be analyzed daily from day of admission to discharge (day 7) to investigate how the antioxidant capacity varies during different stages of the infection. Serum will also be analyzed on day 14 of convalescence stage] | — |
Secondary
| Measure | Time frame |
|---|---|
| Serum Bilirubin- Diazo method – manually performed using colorimetric assay kit [Serum samples will be analyzed daily from day of admission to discharge (day 7) to investigate the variations of the serum antioxidant bilirubin during different stages of the infection. ] Serum Uric acid - Uricase method- manually performed using colorimetric assay kit [ Serum samples will be analyzed daily from day of admission to discharge (day 7) to investigate the variations of the serum antioxidant uric acid during different stages of the infection. ] Serum albumin - Bromocresol Green Method (BCG) manually performed using colorimetric assay kit [ Serum samples will be analyzed daily from day of admission to discharge (day 7) to investigate the variations of the serum antioxidant albumin during different stages of the infection. ] | — |
Countries
Sri Lanka
Contacts
Public ContactProf. V. Pujitha Wickramasinghe
Professor in Paediatrics
Outcome results
None listed