Not applicable. The aim is to establish the catabolic and absorption profile of cocoa flavanols catabolites generated by the human colonic microbiota.
Conditions
Interventions
The intervention will consist of daily consumption of 20 grams of dark chocolate for five days (5 days). In the beginning, the participant will provide a stool sample (in order to establish the baseli
Sponsors
Compañía Nacional de Chocolates SAS
Eligibility
Sex/Gender
All
Age
18 Years to 50 Years
Inclusion criteria
Inclusion criteria: 1.Male and female, 2. between 18 and 50 years old, 3. habitual consumers of products derived from cocoa such as chocolate, cocoa, among others. 4. Non-smokers. 5. No diagnosis of gastrointestinal diseases. 6. No consumption of antibiotics, antiparasitic, and/or laxatives in the last three months. 7. Non-vegetarian. 8. Not be enrolled in weight loss programs or consuming weight loss supplements.
Exclusion criteria
Exclusion criteria: 1. Pregnant or breastfeeding women. 2. High-performance athletes.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Catabolites in stool samples (gas chromatography/mass spectrometry; colorimetric methods). Measurement time: baseline and 5 days after chocolate consumption. 2. Catabolites in urine samples (gas chromatography/mass spectrometry; colorimetric methods). Measurement time: baseline (occasional urine first in the morning), 10 hours after the first consumption, and 5 days after the consumption of chocolate. 3. In vitro, microbial catabolism will be evaluated on a fraction of flavanols (gas chromatography/mass spectrometry; colorimetric methods). Measurement time: 0, 3, and 6 hours. | — |
Secondary
| Measure | Time frame |
|---|---|
| Composition of the microbiota (Operational Taxonomic Units, defined to 97% similarity of DNA sequences of variable regions of the 16S rRNA gene). Measurement time: baseline and 5 days after chocolate consumption. | — |
Countries
Colombia
Contacts
Public ContactVanessa Corrales-Agudelo
Vidarium, Nutrition, Health and Wellness Research Center
Outcome results
None listed