Diabetes mellitus, denture Stomatitis
Conditions
Interventions
All patients receive the same cleaning protocol to the palate and denture, differing only the solutions among the groups, during 37 days.
Stratum 1 (4 groups): healthy patients: Control group: 15 Tot
Other
E06.761.726.794
Sponsors
Faculdade de Odontologia de Ribeirão Preto FORP/USP
Faculdade de Odontologia de Ribeirão Preto FORP/USP
Eligibility
Inclusion criteria
Inclusion criteria: Patients with stomatitis Prosthetics; use of conventional dentures; base and artificial teeth in acrylic resin; at least use time one year; appropriate conditions of prostheses; presence of biofilm on the inner surface of the prosthesis.
Exclusion criteria
Exclusion criteria: Prostheses with problems in adaptation; reline; repair or fractures; Use of antifungals; antibiotics or steroids in the past 3 months; anemia; immunosuppression or cancer therapy; other immunosuppressive diseases.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Assess the virulence factors of different Candida species present in the denture and palate after using denture cleansers for soaking dentures. the samples positive are selected for evaluation of virulence factors: adhesion capacity on the heat acrylic resin by epifluorescence microscopy and viability of the biofilm formation using the XTT method and microbial / counting culture; Proteinase activity and phospholipase by employing specific kits for such formation of hyphae, and a specific culture media and inducing medium. | — |
Secondary
| Measure | Time frame |
|---|---|
| Identify Candida species present in the biofilm of the palate and denture, using Chromagar microbial culture and PCR, polymerase chain reaction.;Comparison between healthy and diabetic patients type II, both with stomatitis using the Candida strains isolated in vivo for analysis of experimental solutions used in baseline, seven and thirty days. The analysis will be performed by means of test results of virulence factors | — |
Countries
Brazil
Contacts
Public ContactClaudia;Mauricio Silva-Lovato;Badaro
Faculdade de Odontologia de Ribeirão Preto FORP/USP;Faculdade de Odontologia de Ribeirão Preto FORP/USP
Outcome results
None listed