Muscle fatigue
Conditions
Interventions
Experimental group: 80 volunteers will receive cold water immersion in water with temperature of 5 or 10 degrees Celsius for 5 or 10 minutes, in a according to randomic decision.
Control group: 20 vol
Other
E02.258
Sponsors
Universidade Federal de Mato Grosso do Sul
Universidade Federal de Mato Grosso do Sul
Eligibility
Sex/Gender
Male
Age
18 Years to 35 Years
Inclusion criteria
Inclusion criteria: Healthy; male; aged between 18 and 35 years; students in undergraduate or postgraduate public higher education institutions or private.
Exclusion criteria
Exclusion criteria: Presence of musculoskeletal injury in dominant leg in the last two months; Raynaud's disease; allergies to cold; labor or physical activity that requires effort level in intensity that makes it impossible to volunteer at the time of survey participation is in muscular condition of homeostasis.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The expected primary outcome is that recovery in muscle performance is higher with cold water immersion (CWI) than passive recovery (PR). The muscle performance will be assessed by maximal isometric force (MIF), in kilogram-force (kgf), identified from a maximal isometric voluntary contraction (MIVC) test performed before and after an exhaustion test in submaximal intensity (40 or 80 %MIF). For this purpose a load cell will be used. Under these conditions, it is observed reduced levels of maximal force. The recovery of the MIF will be stimulated by means of CWI, with variations on temperature and immersion time. 15 and 30 minutes after the exhaustion again the MIF will be evaluated to check their return to pre-exhaustion levels. In the same times that the MIF was evaluated, it will also be collected electromyographic (EMG) activity of the vastus lateralis, rectus femoris and vastus medialis muscles using surface electromyography. A load cell and an electromyography device (Miotec Biomedical Equipment) with calibration of gain of 1000, sampling frequency of 2000 Hz, high-pass filter of 10 Hz, low-pass filter of 500 Hz and notch-filter 60 Hz. The software used to evaluate the EMG signal and muscle force will be Miotec Suite. Root mean square (RMS), in microvolts, and median frequency (MF), in hertz (Hz), values will be obtained from EMG signal. The increase in RMS values and the decrease in MF values after exhaustion are classic evidence of the occurrence of muscle fatigue, while the return of these values to pre-exhaustion values are indicative of recovery of muscle function. Finally, also for evaluation of muscle function, successive RMS and FM values obtained every 0.5 second of recorded EMG activity of exhaustion test will be correlated with exhaustion time using simple linear regression model. This procedure allows to obtain the degree of increase of the electrical activity of muscles (RMS versus time) or decrease of the firing rates of motor neurons(MF versus ti | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary outcomes expected are related to additional variables investigated in this study. They are: perceived exertion (PSE), delayed onset muscle soreness (DOMS) and heart rate variability (HRV). For the PSE will use the Borg CR-10 scale, a scale in which volunteers verbally express their perceived effort. This will occur immediately after, 15 and 30 minutes after the exhaustion test. In these same times, in order to capture the RR-intervals parameters, for analysis of heart rate variability (HRV), a chest belt will be placed over the precordium region and a heart rate receiver (Polar V800 - Polar Electro, Finland). HRV analysis will be performed from RR-intervals series at a sampling rate of 1000 Hz. Digital filtering will be supplemented by manual filtering in order to remove the artifacts, ectopic and premature beats. The extraction of each cardiac period (RR-interval) will be analyzed using Kubios HRV Analysis Software. For analysis in time domain, mean RR, RMSSD, e SDDN indices will be analyzed, expressed in milliseconds. In the frequency domain the spectral components of very low frequency (VLF), low frequency (LF) and high frequency (HF) will be used. For the quantitative analysis of the Poincaré plot (PP), SD1 and SD2 will be utilized. Finally, the DOMS will be assessed using a visual analog scale (VAS) with 10 cm, with 0 indicating the absence and 10 maximum pain intensity. The scaling will be applied immediately, 24 and 48 hours after exhaustion test. For PSE and VAS, the reduction of these indices after cold water immersion (CWI) are expected, ie, reduced or absent levels of effort and pain perceived. In the case of HRV are expected positive effects of CWI in restoring cardiac autonomic modulation, with the indeces related to this analysis being reinstated in the post intervention for recovery assessments. | — |
Countries
Brazil
Contacts
Public ContactSilvio Oliveira Júnior
Universidade Federal de Mato Grosso do Sul
Outcome results
None listed