Oxidative Stress
Conditions
Interventions
Study 1: Thirty health subjects participated in this randomized crossover trial, which were randomized according to a simple random sampling in two groups: Juçara Group, which received juçara juice (E
Sponsors
Universidade Federal de Santa Catarina - UFSC
Universidade Federal de Santa Catarina - UFSC
Eligibility
Age
19 Years to 59 Years
Inclusion criteria
Inclusion criteria: Age between 19 e 59 anos years; Body Mass Index (BMI) between 18.5 e 24.9 kg/m².
Exclusion criteria
Exclusion criteria: Not smoke; not drink alcohol regularly (maximum allowed 1 drink/week, e.g. 1 beer can or 150 mL of wine or 45 mL of distilled beverage); not use medicines or dietary supplements; not pregnant; no clinical condition as cardiovascular, endocrine, gastrointestinal, renal or hepatic diseases; no infections or inflammatory processes visible or, known three months prior to the study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To evaluate the biomarker Total Antioxidant Capacity (TAC), according to the method described by Erel (2004), considering a significant difference of 10%, after intervention.;To evaluate the biomarker Oxidative Stress Index (OSI), calculated according to the following formula: OSI (arbitrary unit) = TOS (µmol H2O2 eqv./L)/TAC (mmol trolox eqv./L) (CINGI YIRÜN et al. (2016).;To evaluate the biomarker glutathione peroxidase (GPx), according to the method described by Wendel (1981), considering a significant difference of 10%, after intervention.;To evaluate the biomarker superoxide dismutase (SOD), measured with a Sigma-Aldrich® SOD assay kit (Saint Louis, Missouri, USA) following the methodology of Peskin and Winterbourn (2000), considering a significant difference of 10%, after intervention.;To evaluate the biomarker catalase (CAT), according to the method described by Johansson & Borg (1988), considering a significant difference of 10%, after intervention.;To evaluate the biomarker uric acid, evaluated by the Trinder method, according to instructions of the manufacturer Labtest® (Lagoa Santa, Minas Gerais, Brazil).;To evaluate the biomarker total cholesterol, evaluated by the Trinder method, according to instructions of the manufacturer Labtest® (Lagoa Santa, Minas Gerais, Brazil), considering a significant difference of 10%, after intervention.;To evaluate the biomarker High Density Lipoprotein cholesterol (HDL-c), evaluated by method of precipitation of lipoproteins (Labtest®, Lagoa Santa, Minas Gerais, Brazil), considering a significant difference of 10%, after intervention.;To evaluate the biomarker Low Density Lipoprotein cholesterol fraction (LDL-c), calculated by the Friedewald formula [LDL-c = total cholesterol - (HDL-c + triglycerides/5)] (FRIEDEWALD, LEVY, FREDRICKSON, 1972), considering a significant difference of 10%, after intervention.;To evaluate the biomarker triglycerides, evaluated by the Trinder method, according to instructions of the manufactu | — |
Secondary
| Measure | Time frame |
|---|---|
| To evaluate the diet quality of the subjects, using the food intake assessment instrument Dietary Total Antioxidant Capacity (DTAC) (CARLSEN et al., 2010; PUCHAU et al., 2009). This instrument make it possible to evaluate the dietary antioxidant capacity, so that it is expected to verify possible correlations between the biomarkers and the habitual food intake of the subjects.;To evaluate the diet quality of the subjects, using the dietary index Brazilian Healthy Eating Index Revised (BHEI-R) (ANDRADE et al., 2013; PREVIDELLI et al., 2011). This instrument make it possible to evaluate the overall diet quality, making it possible to widely evaluate food intake, so that it is expected to verify possible correlations between the biomarkers and the habitual food intake of the subjects. | — |
Countries
Brazil
Contacts
Public ContactPatricia Di Pietro
Universidade Federal de Santa Catarina - UFSC
Outcome results
None listed