Chronic Kidney Disease
Conditions
Interventions
Nine patients underwent control protocol and the intervention protocol (n=9). The control protocol (n=9) consists of performing a hemodialysis session as routine service. In the intervention protocol,
Other
E02.779.483
Sponsors
Centro Universitário Metodista - IPA
Hospital Ernesto Dornelles
Eligibility
Age
45 Years to 70 Years
Inclusion criteria
Inclusion criteria: Patients with chronic kidney disease on hemodialysis three times a week, for more than six months; both genders, aged 45 to 70 years.
Exclusion criteria
Exclusion criteria: Severe heart diseases; amputation of the lower limbs; cognitive disorders; inability to understand the guidelines to carry out the tests proposed; musculoskeletal and osteo disabling; presence of femoral fistula.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Primary outcome expected: increased c-reactive protein after exercise intradialítico. C-reactive protein was analyzed by turbidimetry technique automated apparatus BS 120 (Bioclin, MG, Brazil). Analyses were performed following the procedures recommended by the manufacturer. About eight ml of peripheral blood was collected through access for hemodialysis in heparinized tubes for obtaining whole blood at times before, during and immediately after each experimental session. C-reactive protein was presented in mg / L, expected to have a statistically significant increase (p <0.05) of their absolute value after the intervention.;Primary outcome found: The kinetics of reactive c protein during the evaluated times did not differ significativa.Os values obtained for C-reactive protein before, during and after the intervention (intradialítico exercise) were 5.02 ± 2,34mg / L, 4 45 ± 2,53mg / l 5.3 ± 2,27mg / l respectively. In the control protocol values were 7.08 ± 3.64 mg / L 5.85 ± 3,36mg / l 8.38 ± 4.20 mg / L, respectively. No statistical differences were observed in comparisons before and after intraprotocolo and between protocols. | — |
Secondary
| Measure | Time frame |
|---|---|
| Expected secondary outcome: increase in natural killer cells, creatine and CC chemokine type 5. The serum levels of CK were performed by spectrophotometry in the automated device BS 120 (Bioclin, MG, Brazil). Immunophenotyping was performed using a direct immunofluorescence technique. Monoclonal antibodies specific for CD3 + CD16-CD56 + (NK cells) and CD195 (CCR5) and the appropriate isotype controls were purchased from Becton Dickinson (San Jose, California, USA). It used the method of staining in two colors, using monoclonal antibodies labeled with fluorescein isothiocyanate (FITC) and phycoerythrin (PE) added to 12 x 75 mm test tubes. The data of these parameters were presented in frequency (percentage%).;Secondary outcome found: there was no significant difference in the frequency of peripheral CC chemokine type 5 or natural killer. The kinetics of creatine during the evaluated times showed no significant difference. Comparing the moments before, during and after hemodialysis, the intervention protocol showed reduction in peripheral frequency of CCR5 chemokine (11.6 ± 1.18%, 10.55% ± 1.9 and 9.0 ± 1.41% ) with statistically significant difference between the moments before and after hemodialysis (p = 0.018), but no significant reduction in the frequency of peripheral natural killer (19.49 ± 5.32%, 16.48 ± 3.8%; 18, 77 ± 3.8%). The control protocol showed no significant difference in the peripheral frequency of CCR5 chemokine (6.8 ± 2.5%, 6.2% ± 1.2, 5.8 ± 2%) or natural killer cell (16.48 ± 4, 32%; 12.14% ± 3.6, 9.5 ± 2.1%). | — |
Countries
Brazil
Contacts
Public ContactMariane Monteiro
Centro Universitário Metodista - IPA
Outcome results
None listed