dental caries
Conditions
Interventions
An in situ, crossover and double-blind study was carried out during two experimental phases of 14 days each. Sixteen adult volunteers were randomized regarding the use of fluoride toothpaste containin
Drug
Behavioural
Other
Sponsors
Faculdade de Odontologia da UFRGS
Faculdade de Odontologia da UFRGS
Eligibility
Inclusion criteria
Inclusion criteria: undergradueted and gratueted students in Dentistry at UFRGS;both genders; a favorable systemic health condition; not having used antibiotics in the 2 months preceding the study.
Exclusion criteria
Exclusion criteria: have an unfavorable systemic health condition; have used antibiotics during the 2 months preceding the study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| To evaluate the remineralization of dental enamel with artificial caries lesion after the use of arginine-containing dentifrice. This was done using the surface microhardness method, which allowed calculating the percentage of surface hardness recovery (% RDS) at the end of the study. This result indirectly expresses the extent to which the carious lesion has regressed. It was observed that the% RDS (mean ± standard error) of the group that used the toothpaste containing arginine (26.82 ± 4.25) was statistically similar to the group that used conventional toothpaste without arginine (24.09 ± 4.25). | — |
Secondary
| Measure | Time frame |
|---|---|
| Evaluate the biomass of the dental biofilm formed at the end of each experimental phase. The biofilms formed on the dental blocks were collected and weighed on an analytical balance. It was observed that the weight (mean ± standard error; mg) of the biofilms formed in the presence of toothpaste containing arginine (36.69 ± 8.51) was statistically similar to the group used conventional toothpaste without arginine (30.55 ± 5.96).;Evaluate the count of total bacteria present in the dental biofilm formed at the end of each experimental phase. The biofilms formed on the dental blocks were collected, processed in the laboratory and aliquots were inoculated in culture medium. The number of colony forming units (CFU) in each condition was determined. It was observed that the amount of bacteria (log of mean ± standard error; UFC/mg) in biofilms formed in the presence of toothpaste containing arginine (5.88 ± 0.51) was statistically similar to the group of used conventional toothpaste without arginine (6.46 ± 0 , 21).;Evaluate the fluoride concentration present in the dental biofilm formed at the end of each experimental phase. The biofilms formed on the dental blocks were collected, processed in the laboratory and the fluoride present in the biofilms was analyzed with a specific ion electrode. It was observed that the fluoride concentration (mean ± standard error; ug / g) in biofilms formed in the presence of toothpaste containing arginine (391.46 ± 97.00) was statistically similar to the group of used conventional toothpaste without arginine (515.26 ± 119.94).;Assess the concentration of calcium present in the dental biofilm formed at the end of each experimental phase. The biofilms formed on the dental blocks were collected, processed in the laboratory and the calcium present in the biofilms was determined based on the colorimetric method using a spectrophotometer. It was observed that the calcium concentration (mean ± standard error; uM / mg) in biofilms formed in the pre | — |
Countries
Brazil
Contacts
Public Contactmaria eduarda ;rodrigo pagnussatti ;arthur
Faculdade de Odontologia da UFRGS ;Faculdade de Odontologia da UFRGS
Outcome results
None listed