Colitis
Conditions
Interventions
Oral antibiotic therapy will be performed for 3 days with neomycin 500 mg/3 times a day, metronidazole 500 mg/3 times a day and vancomycin 125 mg/4 times a day. Antibiotics-treated subjects (10 subjec
Sponsors
Instituto de Biologia - Universidade Estadual de Campinas
Instituto de Biologia - Universidade Estadual de Campinas
Eligibility
Sex/Gender
Male
Age
20 Years to 29 Years
Inclusion criteria
Inclusion criteria: Male, volunteer subjects; age between 20 and 29 years; eutrophic (Body Mass Index between 18.5 and 29.9); non smokers; in good general health.
Exclusion criteria
Exclusion criteria: Recent (up to 60 days before treatment) history of: antibiotic or corticosteroid therapy; vaccination; continuous drug therapy. Diagnosis or clinical signs of: metabolic diseases; acute or chronic inflammatory diseases ; autoimmune diseases; allergy or atopy; cancer; digestive system diseases; acute or chronic infectious disease known to impair general health or immune responses (for examples: HIV infection, hepatitis C, influenza, acute herpes simplex); depression, hypochondria, or other psychiatric illnesses that may lead to risky behaviors. Not mentioned conditions that may interfere with the study will follow medical advice regarding exclusion or permanency in the study.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary endpoint of this study is the list of differentially expressed genes (DEG) in in vitro stimulated or unstimulated neutrophils from treated and untreated subjects. Such gene list is obtained by transcriptome sequencing (RNAseq) followed by bioinformatics data analysis and statistical treatment. | — |
Secondary
| Measure | Time frame |
|---|---|
| Oral administration of antibiotics to healthy subjects is expected to promote temporary disruption of the gut microbiota (dysbiosis). Such an outcome will be evaluated by identifying the amount and type of microorganism found in stool samples (by 16S rRNA). Stool samples will be obtained from antibiotics-treated subjects (before and after treatment), or not treated. | — |
Countries
Brazil
Contacts
Public ContactMarco Vinolo
Universidade Estadual de Campinas - UNICAMP
Outcome results
None listed