Oxidative stress
Conditions
Interventions
Fifteen subjects participated in this randomized and cross-over trial, which were randomized according to a sequence of random numbers, obtained through an online random number generator (www.randomiz
Dietary supplement
G11.427.410.698.277.311.250
Sponsors
Universidade Federal de Santa Catarina - UFSC
Universidade Federal de Santa Catarina - UFSC
Eligibility
Sex/Gender
Male
Age
19 Years to 30 Years
Inclusion criteria
Inclusion criteria: Male; age between 19 and 30 years; and physically active
Exclusion criteria
Exclusion criteria: Sedentary individuals; smokers; carriers of any diseases, visible or known inflammatory or infectious processes; muscleskeletal injuries in the last 3 months; users of vitamin and mineral supplements, ergogenic resources (carnitine, arginine, creatine and caffeine), anabolic steroids or medications in the month prior to the interventions
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| It is expected to find an improvement of the oxidative stress, evaluated through the biomarkers: - Total phenols, evaluated using the colorimetric method of Folin-Ciocalteau, as described by Serafini, Maiani and Ferro-Luzzi (1998); - Uric acid, evaluated by the Trinder method, according to the instructions of the manufacturer Labtest® (Lagoa Santa, Minas Gerais, Brazil); - Reduced glutathione (GSH) and oxidized glutathione (GSSG), evaluated according to the methodology described by Giustarini et al. (2013); - GSH:GSSG ratio, calculated according to the following formula: GSH (µmol/g/hb)/GSSG (mmol/g/hb); - Total Antioxidant Capacity (TAC), evaluated according to the method described by Erel (2004); - Total Oxidant Status (TOS), evaluated according to the method described by Erel (2005); - Oxidative Stress Index (OSI), calculated according to the following formula: OSI (arbitrary unit) = TOS (µmol H2O2 eqv./L)/TAC (mmol trolox eqv./L) (Cingi Yirün et al. (2016); - Protein Carbonyls (PC), determined by colorimetric method, as described by Levine et al. (1990); - Glutathione Peroxidase (GPx), evaluated according to methodology proposed by Wendel (1981); - Superoxide dismutase (SOD), measured with a Sigma-Aldrich® SOD assay kit (Saint Louis, Missouri, USA) following the methodology of Peskin and Winterbourn (2000); - Catalase (CAT), measured by its peroxidative function, as described by Johansson and Borg (1988); Considering a significant difference of 9% after intervention (average of the percentage values found for the difference in the studies by Bowtell et al., 2011 and Howatson et al., 2010). Data collected 1 hour prior to the HIIT session, immediately after and 1 hour after the HIIT session. References: BOWTELL, J. L.; SUMNERS, D. P.; DYER, A. et al. Montmorency Cherry Juice Reduces Muscle Damage Caused by Intensive Strength Exercise. Medicine & Science in Sports & Exercise, v. 43, n. 8, p. 1544-1551, 2011. CINGI YIRÜN, M.; ÜNAL, K.; ALTUNSOY SEN, N. et al. Ev | — |
Secondary
| Measure | Time frame |
|---|---|
| The cytokines TNF-a, IL-6, IL-8, IL-1b and IL-10 will be evaluated by the Flow Cytometer (CBA) assay. IL-6 attenuation is expected in the group that consumed juçara juice, since Bell et al. (2016) found a significant reduction of IL-6 in the group that received black cherry (vs. placebo) (p = 0.003), with peak differences of 3.10 pg/mL immediately after exercise (p = 0.03). The same authors observed an increase in plasma IL-8 (p = 0.010) and TNF-a (p < 0.001) at 72 h, and no difference could be found in our study since it was evaluated up to 60 min after exercise. However, our study has an exercise and feeding protocol that has not yet been seen in the scientific literature, bringing new perspectives and more information on inflammation in the exercise context. Data collected 1 h prior the HIIT session, immediately after, 30 min after, and 1 h after the HIIT session. Plasma cortisol will be evaluated by the ELISA method and it is expected to find lower values for the group that consumed juçara juice in relation to the group that consumed water, since the study of Levers and collaborators (2016) supports this hypothesis, since it found levels serum cortisol levels were 44% higher than the pre and post race values for the control group and only 15% increase for the group that consumed cherry. Data collected at the second moment of the study (described in the intervention item). For the depressive and anxiety symptoms, the questionnaires used were: Beck Depression Inventory (IDB) and the Trait-State Anxiety Inventory (IDATE). For the IDB questionnaire the cut points for symptoms of depression are: less than 10 = no symptoms of depression; from 11 to 18 = mild to moderate depression symptoms; from 19 to 29 = moderate to severe depression symptoms; from 30 to 63 = symptoms of severe depression. For the IDATE questionnaire the total score is characterized by low anxiety (20-30), average anxiety (31-49) and high anxiety (50 or higher), according to the study by Barreto | — |
Countries
Brazil
Contacts
Public ContactPatricia Di Pietro
Universidade Federal de Santa Catarina - UFSC
Outcome results
None listed